Dimethyl sulfoxide Hybri-Max™, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%

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Properties

Related Categories	BRDU Cell Proliferation Assay Reagents, Cell Biology, Cell Culture, Cell Freezing Media and Reagents, Cell Signaling and Neuroscience, Chemical ER Chaperones, Core Bioreagents, Cryoprotectants, Dimethyl Sulfoxide (DMSO), Endoplasmic Reticulum Stress (ER Stress), General Reagents, Hybridoma Platform, Hybridoma Reagents, Life Science Reagents for Cell Culture, Reagents and Supplements, Research Essentials, Serum-free Media, Solvent by Type, Solvents More...
Quality Level	PREMIUM
grade	Hybri-Max^™
vapor density	2.7 (vs air)
vapor pressure	0.42 mmHg ( 20 °C)
InChI Key	IAZDPXIOMUYVGZ-UHFFFAOYSA-N
sterility	sterile-filtered
product line	BioReagent
assay	≥99.7%
autoignition temp.	573 °F
expl. lim.	42 %, 63 °F
impurities	endotoxin, tested
	≤0.1% water water
refractive index	n20/D 1.479(lit.)
bp	189 °C(lit.)
mp	16-19 °C(lit.)
solubility	H₂O: miscible (completely)
density	1.10 g/mL(lit.)
suitability	suitable for hybridoma

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Description

Application

DMSO is a polar aprotic solvent used in chemical reactions, in polymerase chain reactions (PCR) and as a cryoprotectant vitrification agent for the preservation of cells, tissues and organs. DMSO is used in cell freezing media to protect cells from ice crystal induced mechanical injury. It is used for frozen storage of primary, sub-cultured, and recombinant heteroploid and hybridoma cell lines; embryonic stem cells (ESC), and hematopoietic stem cells. DMSO is frequently used in the combinations with BSA or fetal bovine serum (FBS).

Caution

Supercools easily and remelts slowly at room temperature. Solidified product can be re-liquified by warming to room temperature without detriment to the product.

Other Notes

This product is a Hybri-Max product. It is hybridoma tested and is assessed for suitability in cell freezing. This product is sterile filtered (0.2 micron) and tested for endotoxin levels.

Packaging

5 mL and 10 mL in flame sealed ampules, 100 mL in amber bottle

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

Personal Protective Equipment

Eyeshields, full-face respirator (US), Gloves, multi-purpose combination respirator cartridge (US), type ABEK (EN14387) respirator filter

RIDADR

NA 1993 / PGIII

WGK Germany

1

RTECS

PV6210000

Flash Point(F)

188.6 °F

Flash Point(C)

87 °C

Documents

Certificate of Analysis

Certificate of Origin

Bulk Quote-Order Product

SDS

Specification Sheet

D2650 - Product Information Sheet (28 KB)

FT-IR Raman

FT-NMR

Pressure-Temperature Nomograph

Structure Search

Frequently Asked Questions

What is the molarity of DMSO?

DMSO will have a molarity of 14.1M, based on a density of 1.1 g/mL and a molecular weight of 78.13 g/mol.

What is the DMSO dissolved in?

DMSO is not dissolved in any solvent. It is a pure chemical compound that is a liquid at room temperature.

Where does DMSO come from?

DMSO is known to have been isolated from tree bark in the past. It is now a commericially synthesized solvent.

What concentration of DMSO do I use in a cell freezing media?

DMSO is usually used at 1-10%, depending on the cell line. For the cell freezing media that we offer, it is used at 8.7% concentration.

Does DMSO have any synthetic utility?

DMSO has been used to oxidize thiols and disulfides to sulfonic acids, hydrolysis of epoxides, thioalkylation of phenols, and oxidation of primary alcohols and halides, and esters of primary alcohols to aldehydes.

Which document(s) contains shelf-life or expiration date information for a given product?

If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

What is the specification for 'pass' for cell freezing on the Certificate of Analysis for Dimethyl sulfoxide (DMSO)?

Product D2438 is assessed for cell freezing. Cells are frozen and stored at -70^°C. The cells are thawed, cultured and viability is assessed at 24 hours. There is no evidence of cytotoxicity or abnormal cell growth.

Dimethyl sulfoxide (DMSO) should be a liquid. Why did it arrive as a solid?

DMSO supercools easily, and re-melts slowly at room temperature. The melting point is 16-19 ^°C. The solidified product can be re-liquefied by warming to room temperature without detriment to the product.

What is the basis for the endotoxin specification for Dimethyl sulfoxide (DMSO)?

The industry standard for 'endotoxin free' is less than 1 EU/mL.

What type of filter is used to sterile-filter Dimethyl sulfoxide (DMSO)?

DMSO can be sterile-filtered through a 0.2 micron PTFE filter. The sterility testing is per the current US Pharmacopoeia method.

How do I get lot-specific information or a Certificate of Analysis?

The lot specific COA document can be found by entering the lot number above under the "Documents" section.

How do I find price and availability?

There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.

What is the Department of Transportation shipping information for this product?

Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

My question is not addressed here, how can I contact Technical Service for assistance?

Ask a Scientist here.

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Protocols & Articles

Articles

Accelerated Osteocyte Differentiation of Human Mesenchymal Stem Cells in a Novel Xeno-free and Serum-free Osteogenesis Differentiation Media

Mesenchymal stem cells (MSCs) have the capacity for multi-lineage differentiation, giving rise to a variety of mesenchymal phenotypes such as osteoblasts (bone), adipocytes (fat), and chondrocytes (c...
Anna Abai, Nick Asbrock and Vi Chu¹
¹ MilliporeSigma, Bioscience Division, Temecula, CA, USA

Keywords: Cell culture, Clinical, Drug discovery, Gene expression, Immunostaining

Pressure-Temperature Calculator for Solvents

*Calculations provide estimated results based on Antoine's Coefficients and their associated temperature range values. Antoine's Coefficients were obtained from the National Institute of Standards an...

Protocols

Cytokinesis-block micronucleus cytome assay

a Genome Health Nutrigenomics Laboratory, CSIRO Human Nutrition, Food Science Australia, PO Box 11141
Keywords: Addition reactions, Amplification, Anticoagulants, Apoptosis, Cancer, Carcinogens, Cell culture, Condensations, Drug discovery, Electronics, Electrophoresis, Eliminations, Environmental, Gel electrophoresis, Genetic, Immunofluorescence, Mass spectrometry, Mutagens, Nutrition, PAGE, Polymorphisms, Rearrangements, Recombination, Sample preparations, Separation, Sterilizations, Teratogens, Vitamins

Induced Pluripotent Stem Cell Reprogramming Protocols

Introduction Methods Reprogramming of Human Fibroblasts using Non-Integrating Self-Replicating RNA Vectors Reprogramming of Peripheral Blood Mononuclear Cells (PBMCs) using STEMCCA Lentiviral...
Keywords: Apoptosis, Cell culture, Culture media, Gene expression, Growth factors, Immunocytochemistry, Polymerase chain reaction, Respiratory, Transduction, Transfection

Mesenchymal Stem Cell Culture Protocols

Introduction Methods Isolation of Mesenchymal Stem Cells Expansion of Human Mesenchymal Stem Cells Preparation of Coated Plates Thawing of Mesenchymal Stem Cells Expansion of Mesenchy...
Keywords: Cell disruption, Clinical, Culture media, Gene expression, Immunocytochemistry, Stem cell biology

Protocol for the Cryopreservation of Cell Lines

Interested in receiving more content like this? Join over 8,000 of your peers who have already signed up for the Cell Informer newsletter.
Keywords: Cryopreservation, Phase transitions

Resuscitation of Frozen Cell Lines

Many cultures obtained from a culture collection, such as ECACC, will arrive frozen and in order to use the cells they must be thawed and put into culture. It is vital to thaw cells correctly in orde...
Fundamental Techniques in Cell Culture Laboratory Handbook - 2nd Edition
Keywords: Centrifugation, PAGE

References

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