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D4822 Sigma-Aldrich

Dulbecco’s Modified Eagle’s Medium - high glucose

HEPES modification, With 4500 mg/L glucose, L-glutamine, 25 mM HEPES, and sodium bicarbonate, without sodium pyruvate, liquid, sterile-filtered, suitable for cell culture

Synonym: DME, DMEM



Quality Level   300
sterility   sterile-filtered
form   liquid
application(s)   cell culture | mammalian: suitable
  cell culture | stem cell: suitable
impurities   Endotoxin, tested
components   sodium pyruvate: no
L-glutamine: yes
phenol red: yes
glucose: high
shipped in   ambient
storage temp.   2-8°C



Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".

Safety & Documentation

Safety Information

NONH for all modes of transport
Protocols & Articles

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