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D5905 Sigma-Aldrich

3,3′-Diaminobenzidine tetrahydrochloride

tablet, 10 mg substrate per tablet

Synonym: 3,3′,4,4′-Biphenyltetramine tetrahydrochloride, 3,3′,4,4′-Tetraaminobiphenyl tetrahydrochloride, DAB

  • Linear Formula (NH2)2C6H3C6H3(NH2)2 · 4HCl

  • Molecular Weight 360.11

  •  Beilstein/REAXYS Number 1212988

  •  EC Number 231-018-9

  •  MDL number MFCD00012969

  •  PubChem Substance ID 24893981

  •  NACRES NA.83

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Properties

Related Categories Alphabetical Listing, Biochemicals and Reagents, Colorimetric, Core Bioreagents, Detection,
Quality Level   200
form   tablet
solubility   water: 1 tablet/15 mL (may contain small insoluble particles)
storage temp.   −20°C
SMILES string   Cl.Cl.Cl.Cl.Nc1ccc(cc1N)-c2ccc(N)c(N)c2
InChI   1S/C12H14N4.4ClH/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8;;;;/h1-6H,13-16H2;4*1H
InChI key   KJDSORYAHBAGPP-UHFFFAOYSA-N

Description

Specificity

Peroxidase substrate suitable for use in immunoblotting and immunohistological staining procedures. This substrate produces an insoluble end product that is brown in color and can be observed visually.

Application

3,3′-Diaminobenzidine tetrahydrochloride has been used for immunohistochemistry.

Reagent for spectrophotometric determination of selenium.

Biochem/physiol Actions

3,3′-Diaminobenzidine tetrahydrochloride is a chromogenic substrate, which is commonly used in immunoperoxidase tests.

Reconstitution

Dissolve 1 tablet in 15 ml of Tris-buffered saline, pH 7.6. Add 12 μl of fresh 30% hydrogen peroxide. DAB and hydrogen peroxide concentration may be adjusted to suit individual applications.

Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
RTECS 
DV8753000
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrat...
BioFiles 2008, 3.4, 6.
Keywords: Enzyme-linked immunosorbent assay, Immunohistochemistry, Western blot

Intro to Detection Methods Used in Molecular Biology

Different detection methods are used to visualize the nucleic acids and specific proteins transferred to membrane support. Southern and northern blotting involves the use of radioactive probes and th...
Keywords: Catalytic combustion detector, Detection methods, Northern blot, Nucleic acid hybridization, Purification, Size-exclusion chromatography, Western blot

Peer-Reviewed Papers
15

References

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