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D6434 Sigma-Aldrich

Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham

With 15 mM HEPES and sodium bicarbonate, without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture

Synonym: DME/F-12, 1:1 mixture

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Properties

Related Categories Apoptosis and Cell Cycle, Cell Biology, Cell Culture, Cell Culture Media, Cell Signaling and Neuroscience,
sterility   sterile-filtered
form   liquid
application(s)   cell culture | mammalian: suitable
impurities   endotoxin, tested
components   phenol red: no
L-glutamine: no
NaHCO3: yes
sodium pyruvate: 0.055 g/L
HEPES: 15 mM
glucose: 3.15 g/L
shipped in   ambient
storage temp.   2-8°C

Description

General description

Pyridoxal, which is an aldehyde, has been replaced with pyridoxine. This modification has been shown to improve the stability of the medium. Suitable for growth of a wide range of cell types in low serum conditions. Absence of phenol red makes this a preferred medium for clonal and stem cell culture.

Reconstitution

Supplement with 0.365 g/L L-glutamine

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Single- & multi-channel pipettes from BRAND

Pipette Controller from Brand
Protocols & Articles

Related Content

Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
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Peer-Reviewed Papers
15

References

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