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  • D7304 - 3,3′-Diaminobenzidine (DAB) Liquid Substrate System tetrahydrochloride

D7304 Sigma-Aldrich

3,3′-Diaminobenzidine (DAB) Liquid Substrate System tetrahydrochloride

  •  NACRES NA.46



General description

3,3′-Diaminobenzidine (DAB) is a chromogenic substrate for peroxidase enzyme and is useful as a staining agent in immunohistological and immunoblotting techniques. The end product is resistant to alcohol therefore, a variety of counterstains and mounting media can be used with the DAB Liquid Substrate System. The DAB Liquid Substrate System provides all the chromogen and buffer/peroxide solutions needed to produce a fast and convenient DAB substrate solution..


3,3′-Diaminobenzidine (DAB) Liquid Substrate System tetrahydrochloride has been used as a substrate:
• in the immunoblot assay carried out to identify the changes in heat shock proteins (Hsps) expressed in the liver
• for visualization of sections after immunostaining
• in immunohistochemistry of mice knee joint sections
• in the immunolabeling of kisspeptin neurons in the preoptic area of camel
It produces a brown precipitate with peroxidase for use in immunohistological and immunoblotting staining procedures. Not recommended for ELISA (microwell) procedures.


Stable at least one year at 2-8 °C.


Each DAB Liquid Substrate System will provide 250 mL of DAB substrate solution.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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10X DAB Liquid Chromogen 25 mL    
ready-to-use buffer/peroxide solution 250 mL    
Safety & Documentation

Safety Information

Signal word 
UN 3316 9
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