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D7442 Sigma-Aldrich

MTP Taq DNA Polymerase

Taq DNA Polymerase, free of DNA contaminants

  •  NACRES NA.55

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Properties

Related Categories Molecular Biology, PCR/Amplification, Specialty PCR Enzymes More...
Quality Level   200
form   liquid
feature   hotstart: no
concentration   5 unit/μL
color   colorless
shipped in   wet ice
storage temp.   −20°C

Description

General description

MTP Taq DNA Polymerase is a recombinant thermostable enzyme from Thermus aquaticus expressed in E. coli and purified using a proprietary process to minimize levels of contaminating DNA. The enzyme has both 5′→3′ DNA polymerase and exonuclease activities, is ∼95 kDa by SDS-PAGE, and has no detectable endonuclease or 3′→5′ exonuclease activities. Each lot of MTP Taq undergoes strict quality control testing to ensure the absence of detectable levels of contaminating DNA.

Contaminating DNA present in most other polymerase preparations often preclude or obscure the accurate interpretation of results, especially when targeting conserved sequences, e.g., bacterial 16S rRNA region. Through Sigma′s proprietary DNA removal methods and strict quality control standards, we can ensure the absence of the most commonly found contaminant DNA. Each lot of MTP Taq is assayed using PCR and primers specific to (1) the conserved region of bacterial 16S rRNA, (2) the Taq expression vector, and (3) the human β-actin gene.

While MTP Taq ensures a high-quality, low contaminant DNA polymerase for reliable PCR amplification, DNA contaminants can be introduced into PCR through a number of other reagents. To further minimize the risk of contaminant DNA during PCR, we include 10× MTP Taq Buffer with each tube of MTP Taq DNA Polymerase. Each lot of 10× MTP Taq Buffer undergoes the same strict quality control testing as MTP Taq DNA polymerase to ensure the absence of contaminating DNA. To prevent false positive PCR results, only DNA-free reagents should be used in PCR reactions with MTP Taq DNA polymerase.

Application

MTP Taq DNA Polymerase has been used:
• for the amplification of bacterial 16S rRNA genes from purified DNA(33)
• bacterial genome analysis
• pathogen detection

MTP Taq DNA Polymerase is a recombinant thermostable enzyme from Thermus aquaticus expressed in E. coli and purified using a proprietary process that minimizes levels of contaminating DNA. The enzyme has 5′-3′ DNA polymerase and exonuclease activities, is approximately 95 kD by SDS-PAGE, and has no detectable endonuclease or 3′-5′ exonuclease activities. Contaminating DNA present in most other polymerase preparations often precludes or obscures the accurate interpretation of results, especially when targeting conserved sequences (e.g. bacterial 16S rRNA region).

While MTP Taq is a high-quality, low-contaminant DNA polymerase for reliable PCR amplification, DNA contaminants can be introduced into PCR through a number of other reagents. To further minimize the risk of contaminant DNA during PCR, we include 10× MTP Taq buffer (Sigma product M 9943) with each tube of MTP Taq DNA Polymerase. Each lot of MTP Taq and 10× MTP Taq buffer undergoes the same strict quality control testing to ensure the absence of contaminating DNA. To prevent false positive PCR results, only DNA-free reagents should be used in PCR reactions with MTP Taq DNA Polymerase.

Biochem/physiol Actions

MTP Taq polymerase catalyzes oligonucleotide primer-driven, DNA template dependent incorporation of dNTPs into complimentary DNA strands.

Features and Benefits

Low contaminant DNA polymerase
Prevents false positive PCR results from contaminating bacterial DNA

Components

• MTP Taq DNA Polymerase (D7067)
• 10x MTP Taq Buffer (M9943)

Unit Definition

One unit incorporates 10 nmol of total deoxyribonucleoside triphosphates into acid precipitable DNA in 30 minutes at 74 °C.

Other Notes

Learn more about our offering of specialty enzymes at www.sigma-aldrich.com/specialtyenzymes.

Store at –20 °C. For convenience, 10x MTP Taq Buffer can be stored at room temperature.

View more detailed information on MTP Taq DNA Polymerase at www.sigma-aldrich.com/mtptaq.

Legal Information

No license is conveyed with the purchase of this product under any of US Patents Nos. 5,804,375, 5,994,056, 6,171,785, 6,214,979, 5,538,848, 5,723,591, 5,876,930, 6,030,787, and 6,258,569, and corresponding patents outside the United States, or any other patents or patent applications, relating to the 5′ Nuclease and dsDNA-Binding Dye Processes. For further information contact the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

MTP is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Protocols

Low Contaminant Amplification of DNA Using MTP Taq DNA Polymerase D7442 Protocol

Every precaution should be taken to avoid contamination of reagents with unknown/unwanted DNA. This includes the following:
Keywords: AGE, Amplification, Buffers, Electrophoresis, Evaporation, Gas chromatography, Gel electrophoresis, Nucleic acid annealing, Nucleic acid denaturation, Polymerase chain reaction

PCR amplification of Bacterial DNA with low contamination using MTP™ Taq DNA Polymerase

MTP™ Taq DNA Polymerase is a recombinant thermostable enzyme from Thermus aquaticus expressed in E. coli and purified using a proprietary process to minimize levels of contaminating DNA. The enzyme h...
Keywords: AGE, Amplification, Electrophoresis, Evaporation, Gel electrophoresis, Gene expression, PAGE, Polymerase chain reaction

Related Content

PCR Selection Guide

We offer a wide variety of PCR enzymes, master mixes, and PCR protocols to meet your experimental needs for routine PCR, qPCR, or RT-PCR. Our PCR Selection Guide features various filters to sort by, ...
Keywords: Genomics, Polymerase chain reaction, Polymerase chain reaction - quantitative

Peer-Reviewed Papers
15

References

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