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D7777 Sigma-Aldrich

Dulbecco’s Modified Eagle’s Medium - high glucose

With 4500 mg/L glucose, L-glutamine, and sodium pyruvate, without sodium bicarbonate, powder, suitable for cell culture

Synonym: DME, DMEM

  •  NACRES NA.75

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Properties

Related Categories Apoptosis and Cell Cycle, Cell Biology, Cell Culture, Cell Culture Media, Cell Signaling and Neuroscience,
form   powder
application(s)   cell culture | mammalian: suitable
components   L-glutamine: yes
HEPES: no
NaHCO3: no
glucose: high
sodium pyruvate: yes
phenol red: yes
shipped in   ambient
storage temp.   2-8°C

Description

Application

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".

Quantity

Formulated to contain 13.5 grams of powder per liter of medium.

Reconstitution

Supplement with 3.7 g/L sodium bicarbonate

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Single- & multi-channel pipettes from BRAND

Pipette Controller from Brand
Protocols & Articles

Protocols

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture. The entire contents of each package should be used immediately after opening. Preparing m...

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Peer-Reviewed Papers
15

References

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