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  • D9780 - Lambda DNA Hind III Digest

D9780 Sigma-Aldrich

Lambda DNA Hind III Digest

for DNA electrophoresis

  •  NACRES NA.25



Related Categories DNA Digest Markers, Markers and Ladders, Molecular Biology, Nucleic Acid Electrophoresis
Quality Level   100
grade   for molecular biology
form   liquid
usage   100 uses (0.1 mg size)
  1000 uses (1 mg size)
  20 uses (20 μg size)
  500 uses (0.5 mg size)
suitability   suitable for electrophoresis (DNA)
shipped in   dry ice
storage temp.   −20°C


General description

Sigma’s Lambda DNA Hind III Digest contains 8 fragments from 125 – 23,130 bp. Ten ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.


Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.


Sigma’s Lambda DNA Hind III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Other Notes

Contains 8 fragments, 125–23,130 bp

For optimal resolution, the recommended agarose gel concentration is 0.75%.

Preparation Note

Bacteriophage lambda DNA was digested with EcoR I and Hind III to completion, then purified by phenol extraction.

Safety & Documentation

Safety Information

NONH for all modes of transport


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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