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D9780 Sigma-Aldrich

Lambda DNA Hind III Digest

for DNA electrophoresis

  •  NACRES NA.25

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Properties

Related Categories DNA Digest Markers, Markers and Ladders, Molecular Biology, Nucleic Acid Electrophoresis
Quality Level   100
  200
grade   for molecular biology
form   liquid
usage   100 uses (0.1 mg size)
  1000 uses (1 mg size)
  20 uses (20 μg size)
  500 uses (0.5 mg size)
suitability   suitable for electrophoresis (DNA)
shipped in   dry ice
storage temp.   −20°C

Description

General description

Sigma’s Lambda DNA Hind III Digest contains 8 fragments from 125 – 23,130 bp. Ten ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.

Application

Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.

Components

Sigma’s Lambda DNA Hind III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Other Notes

Contains 8 fragments, 125–23,130 bp

For optimal resolution, the recommended agarose gel concentration is 0.75%.

Preparation Note

Bacteriophage lambda DNA was digested with EcoR I and Hind III to completion, then purified by phenol extraction.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Introduction to Markers and Ladders

We offer a variety of markers that aid size determination of samples separated by agarose and/or polyacrylamide gel electrophoresis. These products include markers for DNA, PCR fragments and RNA and ...
Keywords: Digestions, Electrophoresis, Gel electrophoresis, Molecular biology, PAGE, Polymerase chain reaction, Proteomics

Markers and Ladders Selection Guide

DNA and RNA size markers contain a mixture of DNA (or RNA) fragments of known length, making them suitable for estimating the fragment length of concurrently run samples. They stain well with ethidiu...
Keywords: AGE, Electrophoresis, Gel electrophoresis, PAGE, Polymerase chain reaction

Nancy-520 for DNA Detection and Quantitation

Nancy-520 is a fluorescent stain for double stranded DNA (dsDNA) on agarose electrophoresis gels, with higher sensitivity than ethidium bromide and an easy, fast, and robust staining procedure. It is...
Monika Baeumle
BioFiles 2009, 4.1, 4.
Keywords: Catalytic combustion detector, Electrophoresis

Protocols

GenElute™ Blood Genomic DNA Kit Protocol (NA2010, NA2020)

Our GenElute™ Blood Genomic DNA Kit provides a simple and convenient way to isolate pure genomic DNA from fresh or aged (older than 24 hours) whole blood. The kit combines the advantages of silica bi...
Keywords: AGE, Addition reactions, Amplification, Anticoagulants, Cell disruption, Centrifugation, Digestions, Electrophoresis, Evaporation, Gel electrophoresis, Molecular biology, Nucleic acid denaturation, Phase transitions, Polymerase chain reaction, Precipitation, Purification, Sequencing

Long and Accurate PCR Amplification of DNA with RedAccuTaq D4812 Protocol

Reaction Optimization Reliable amplification of long DNA sequences requires: 1) effective denaturation of DNA template, 2) adequate extension times to produce large products and 3) protection of targ...
Keywords: AGE, Amplification, Buffers, Diagnostic, Electrophoresis, Evaporation, Gas chromatography, Gel electrophoresis, Melting, Nucleic acid annealing, Nucleic acid denaturation, Phase transitions, Polymerase chain reaction, Size-exclusion chromatography, Solvents

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