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E2886 Sigma-Aldrich

ExtrAvidin®−Peroxidase

buffered aqueous solution

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Properties

Related Categories Antibodies, Antibody Conjugates, Avidin / Biotin Reagents, Avidin-Biotin Complex Reagents, Biotin/Avidin,
form   buffered aqueous solution
application(s)   direct ELISA: 1:2,000
  dot blot: 1:4,000
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100
conjugate   peroxidase conjugate
shipped in   wet ice
storage temp.   2-8°C

Description

Specificity

The product is found to be reactive with Anti-Avidin, Catalog Number A5170, and non-reactive with Anti-Streptavidin, Catalog Number S6390, by immunoelectrophoresis.

Application

ExtrAvidin®-Peroxidase is suitable for the following applications:
• Dot blot at a minimum dilution of 1:4,000
• Immunohistochemistry (using formalin-fixed, paraffin-embedded sections at a dilution of 1:100)
• Direct ELISA at a minimum dilution of 1:2,000
It has also been used for:
• Immunoprecipitation and Immunobloting
• Ra2I solid-phase binding assays
• Southwestern blotting
• Western blotting
• Immunocytochemistry

Biochem/physiol Actions

Avidin is a tetrameric or dimeric biotin-binding protein produced in the oviducts of birds, reptiles and amphibians and deposited in the whites of their eggs. It consists of four high affinity binding sites for biotin. ExtrAvidin® is prepared from egg white avidin. It is a modified form of affinity purified avidin with high specific activity of avidin and low background staining of streptavidin. It is a biotin binding protein produced by the bacteria Streptomyces avidinii. ExtrAvidin-Peroxidase may be used in conjunction with biotin in different techniques.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT

Preparation Note

Affinity purified protein

Storage and Stability

Store at 2-8 °C. Do Not Freeze. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Legal Information

ExtrAvidin is a registered trademark of Sigma-Aldrich Co. LLC

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Single- & multi-channel pipettes from BRAND
Protocols & Articles

Articles

Antibodies to Peptides and Fusion Tags

Unlike DNA and RNA, which can be targeted with complementary oligonucleotides, protein detection and purification usually relies upon specific antibodies. Although many are available commercially, no...
Leigh Gaskill
BioFiles 2008, 3.6, 14.
Keywords: Biofiles, Immunofluorescence, PAGE, Purification, Sequences, Western blot

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Immunohistochemistry Protocol

Immunohistochemical staining is a valuable tool for detecting specific antigens in tissues. In order to perform the standard staining procedure, first the tissue section has to be deparaffinized and ...
Keywords: Digestions, Immunohistochemistry, Solvents

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Peer-Reviewed Papers
15

References

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