Preparation of high quality nucleic acids from environmental samples is critical to allow for the use of the purified DNA and RNA in downstream research and diagnostic applications. However, preparat...
Won-Sik Kim¹, and Yousef Haj-Ahmad^1,2
(1)Norgen Biotek Corp., Thorold, ON, Canada, (2)Brock University. St. Catharines, ON, Canada
Keywords: Amplification, Diagnostic, Environmental, Genetic, Polymerase chain reaction, Purification

Frequent failure of downstream applications with DNA and RNA is often related to poor sample preparation. In particular, it is often difficult to extract DNA and RNA from samples that contain high le...
Eric Sonke³, Nezar Rghei¹, Yousef Haj-Ahmad^1,2 and Won-Sik Kim¹
(1) Norgen Biotek Corp. 3430 Schmon Parkway, Thorold, Ontario, Canada, L2V 4Y6, (2) Brock University, 500 Glenridge Avenue, St. Catharines, ON, Canada, L2S 3A1, (3) Department of Anatomy and Cell Biology, Schulich School of Medicine and Dentistry, Western University London, ON, Canada.
Keywords: Amplification, Polymerase chain reaction, Sample preparations, Sequencing

Plum pox virus (PPV) is a serious plant disease affecting tender fruit including peaches, plums, apricots and nectarines, causing significant yield losses. Evidence suggests that the Dideron strain o...
Won-Sik Kim¹ and Yousef Haj-Ahmad^1,2
(1)Norgen Biotek Corp, 3430 Schmon Pkwy, Thorold, ON, L2V 4Y6, (2)Brock University, 500 Glenridge Ave., St. Catharines, ON, L2S 3A1
Keywords: AGE, Amplification, Chromatography, Column chromatography, Diagnostic, Electrophoresis, Enzyme-linked immunosorbent assay, Gel electrophoresis, Polymerase chain reaction, Purification, RNA purification, Sample preparations

Norgen’s Plant/Fungi Total RNA Purification Kit provides a rapid method for the isolation and purification of total RNA from a wide range of plant and filamentous fungal species. Total RNA can be pur...
W.-S. Kim, PhD¹, C. Dobbin, Ph.D¹ and Y. Haj-Ahmad, Ph.D^1,2
(1)Norgen Biotek Corporation, Thorold, Ontario, Canada, (2)Centre for Biotechnology. Brock University, St. Catharines, Ontario, Canada
Keywords: Amplification, Capillary electrophoresis, Cell disruption, Chromatin immunoprecipitation, Chromatography, Column chromatography, Electrophoresis, Enzyme-linked immunosorbent assay, Gel electrophoresis, Gene expression, Genetic, Homogenization, Microarray Analysis, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, RNA purification, Separation, Transcription

Small RNAs, including microRNAs (miRNAs) and short interfering RNAs (siRNAs), are key components of an evolutionarily conserved system of RNA-based gene regulation in eukaryotes. In eukaryotes, regul...
W.-S. Kim, PhD¹, C. Dobbin,PhD¹, Y. Haj-Ahmad, Ph.D^1,2
(1)Norgen Biotek Corporation, Thorold, Ontario, Canada, (2)Centre for Biotechnology. Brock University, St. Catharines, Ontario, Canada
Keywords: Amplification, Capillary electrophoresis, Cell disruption, Centrifugation, Chromatin immunoprecipitation, Chromatography, Column chromatography, Degradations, Electrophoresis, Gel electrophoresis, Gene expression, Homogenization, Lab-on-a-chip, PAGE, Physiological Processes, Polymerase chain reaction, Polymerase chain reaction - quantitative, Precipitation, Purification, RNA purification, Separation, Solvents, Transcription

Determining RNA quality is important prior to performing any downstream expression analysis such as microarrays or RT-qPCR. An RNA sample of poor quality could either lead to a labour-intensive clean...
B. Lam, PhD¹, M. Simkin¹, N. Rghei1, Y. Haj-Ahmad, Ph.D^1,2
(1)Norgen Biotek Corporation, Thorold, Ontario, Canada, (2)Centre for Biotechnology. Brock University, St. Catharines, Ontario, Canada
Keywords: Chromatin immunoprecipitation, Gene expression, Microarray Analysis, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, RNA purification

Norgen’s Plant/Fungi Total RNA Purification Kit provides a rapid method for the isolation and purification of total RNA from a wide range of plant and filamentous fungal species. Total RNA can be pur...
Keywords: Cell disruption, Centrifugation, Chromatography, Column chromatography, DNA purification, Detergents, Diagnostic, Gene expression, Homogenization, Molecular biology, Polymerase chain reaction, Purification, RNA purification, Separation, Transcription