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E4913 Sigma-Aldrich

Plant/Fungi RNA Purification Kit

Green Alternative

sufficient for 50 purifications

  •  NACRES NA.52

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Properties

Related Categories 12 Principles Aligned Products, Chemical Synthesis, DNA & RNA Purification, Greener Alternative Products, Greener Life Science Products,
Quality Level   100
usage   sufficient for 50 purifications
greener alternative product characteristics   Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

Description

General description

Norgen Biotek′s Plant/Fungi Total RNA Purification Kit provides a rapid method for the isolation and purification of total RNA, including viral RNA, from a wide range of plant and filamentous fungal species. Total RNA can be purified from fresh or frozen plant tissues, plant cells or filamentous fungi samples using this kit. All sizes of RNA are purified, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The procedure is rapid and convenient, as it does not rely on the use of liquid nitrogen in order to homogenize the samples.

Application

For the rapid purification of total RNA (including microRNA) from plants and fungi.

Purified RNA is of the highest quality and can be used in a number of downstream applications, including:
• qRT-PCR
• Reverse transcription PCR
• Northern blotting
• RNAse protection
• Primer extension
• Expression array assays
• Next Generation Sequencing

Features and Benefits

• Isolate RNA from a wide range of plant and filamentous fungi samples.
• Robust lysis buffer is suitable for challenging samples such as pine needle or grape leaf.
• Isolate a diversity of RNA species from large mRNA, microRNA, and viral RNA.
• Purification of high yield and quality RNA.
• No phenol/chloroform extraction required.
• Liquid nitrogen is not required for homogenization

Other Notes

Storage Conditions and Product Stability

All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
UN 3316 9

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

A Novel Technology to Extract Nucleic Acids

Preparation of high quality nucleic acids from environmental samples is critical to allow for the use of the purified DNA and RNA in downstream research and diagnostic applications. However, preparat...
Won-Sik Kim1, and Yousef Haj-Ahmad1,2
(1)Norgen Biotek Corp., Thorold, ON, Canada, (2)Brock University. St. Catharines, ON, Canada
Keywords: Amplification, Diagnostic, Environmental, Genetic, Polymerase chain reaction, Purification

Compatibility of DNA and RNA Extraction Methods for Challenging Plant Species

Frequent failure of downstream applications with DNA and RNA is often related to poor sample preparation. In particular, it is often difficult to extract DNA and RNA from samples that contain high le...
Eric Sonke3, Nezar Rghei1, Yousef Haj-Ahmad1,2 and Won-Sik Kim1
(1) Norgen Biotek Corp. 3430 Schmon Parkway, Thorold, Ontario, Canada, L2V 4Y6, (2) Brock University, 500 Glenridge Avenue, St. Catharines, ON, Canada, L2S 3A1, (3) Department of Anatomy and Cell Biology, Schulich School of Medicine and Dentistry, Western University London, ON, Canada.
Keywords: Amplification, Polymerase chain reaction, Sample preparations, Sequencing

Development of a high throughput PPV detection method

Plum pox virus (PPV) is a serious plant disease affecting tender fruit including peaches, plums, apricots and nectarines, causing significant yield losses. Evidence suggests that the Dideron strain o...
Won-Sik Kim1 and Yousef Haj-Ahmad1,2
(1)Norgen Biotek Corp, 3430 Schmon Pkwy, Thorold, ON, L2V 4Y6, (2)Brock University, 500 Glenridge Ave., St. Catharines, ON, L2S 3A1
Keywords: AGE, Amplification, Chromatography, Column chromatography, Diagnostic, Electrophoresis, Enzyme-linked immunosorbent assay, Gel electrophoresis, Polymerase chain reaction, Purification, RNA purification, Sample preparations

Isolation of Plant and Fungal RNA from Challenging Samples using Norgen's Plant/Fungi RNA Purification Kit

Norgen’s Plant/Fungi Total RNA Purification Kit provides a rapid method for the isolation and purification of total RNA from a wide range of plant and filamentous fungal species. Total RNA can be pur...
W.-S. Kim, PhD1, C. Dobbin, Ph.D1 and Y. Haj-Ahmad, Ph.D1,2
(1)Norgen Biotek Corporation, Thorold, Ontario, Canada, (2)Centre for Biotechnology. Brock University, St. Catharines, Ontario, Canada
Keywords: Amplification, Capillary electrophoresis, Cell disruption, Chromatin immunoprecipitation, Chromatography, Column chromatography, Electrophoresis, Enzyme-linked immunosorbent assay, Gel electrophoresis, Gene expression, Genetic, Homogenization, Microarray Analysis, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, RNA purification, Separation, Transcription

Non-Organic-Based Isolation of Plant microRNA using Norgen's Plant/Fungi RNA Purification Kit

Small RNAs, including microRNAs (miRNAs) and short interfering RNAs (siRNAs), are key components of an evolutionarily conserved system of RNA-based gene regulation in eukaryotes. In eukaryotes, regul...
W.-S. Kim, PhD1, C. Dobbin,PhD1, Y. Haj-Ahmad, Ph.D1,2
(1)Norgen Biotek Corporation, Thorold, Ontario, Canada, (2)Centre for Biotechnology. Brock University, St. Catharines, Ontario, Canada
Keywords: Amplification, Capillary electrophoresis, Cell disruption, Centrifugation, Chromatin immunoprecipitation, Chromatography, Column chromatography, Degradations, Electrophoresis, Gel electrophoresis, Gene expression, Homogenization, Lab-on-a-chip, PAGE, Physiological Processes, Polymerase chain reaction, Polymerase chain reaction - quantitative, Precipitation, Purification, RNA purification, Separation, Solvents, Transcription

Revised Guidelines for RNA Quality Assessment for Diverse Biological Sample Input

Determining RNA quality is important prior to performing any downstream expression analysis such as microarrays or RT-qPCR. An RNA sample of poor quality could either lead to a labour-intensive clean...
B. Lam, PhD1, M. Simkin1, N. Rghei1, Y. Haj-Ahmad, Ph.D1,2
(1)Norgen Biotek Corporation, Thorold, Ontario, Canada, (2)Centre for Biotechnology. Brock University, St. Catharines, Ontario, Canada
Keywords: Chromatin immunoprecipitation, Gene expression, Microarray Analysis, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, RNA purification

Protocols

Plant/Fungi Total RNA Purification Kit

Norgen’s Plant/Fungi Total RNA Purification Kit provides a rapid method for the isolation and purification of total RNA from a wide range of plant and filamentous fungal species. Total RNA can be pur...
Keywords: Cell disruption, Centrifugation, Chromatography, Column chromatography, DNA purification, Detergents, Diagnostic, Gene expression, Homogenization, Molecular biology, Polymerase chain reaction, Purification, RNA purification, Separation, Transcription

Peer-Reviewed Papers
15

References

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E5038 Plant/Fungi DNA Isolation Kit, sufficient for 50 purifications

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