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E8033 Sigma

pFLAG-MAC Expression Vector

Bacterial vector for cytoplasmic expression of N-terminal Met-FLAG fusion proteins

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Properties

Related Categories Bacterial Expression Vectors, Cloning and Expression, Molecular Biology, Sigma Expression Vectors
grade   for molecular biology
form   buffered aqueous solution
conjugate   FLAG® tagged
shipped in   dry ice
storage temp.   −20°C

Description

Components

• pFLAG-MAC Expression Vector 10 μg (E5644) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
• pFLAG-ATS-BAP Control Plasmid 1 μg (C9079) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.

General description

The pFLAG-MAC Expression Vector is a 5.0 kb E. coli expression vector used for cloning and cytoplasmic expression of properly inserted open reading frame as an N-terminal Met-FLAG® fusion protein. The Met-FLAG fusion protein may be detected using Monoclonal ANTI-FLAG M2 (F3165), Monoclonal ANTI-FLAG M5 (F4042), and can be purified using the ANTI-FLAG M2 Affinity Gel (A2220).

The pFLAG-ATS-BAP Control Plasmid is a 6.8 kb E. coli plasmid used for efficient and controlled periplasmic expression of N-terminal FLAG-BAP fusion protein. The FLAG-BAP fusion protein may be detected using Monoclonal ANTI-FLAG M1 (F3040), or Monoclonal ANTI-FLAG M2 (F3165), or purified using ANTI-FLAG M1 Agarose Affinity Gel (A4596), or ANTI-FLAG M2 Affinity Gel (A2220).

Vector Maps and Sequences

Legal Information

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

pFLAG-MAC is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles
Peer-Reviewed Papers
15

References

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