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E8283 Sigma

pFLAG-CTS Expression Vector

Bacterial vector for periplasmic expression of C-terminal FLAG fusion proteins

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Properties

grade   for molecular biology
form   buffered aqueous solution
conjugate   FLAG® tagged
shipped in   dry ice
storage temp.   −20°C

Description

Components

• pFLAG-CTS Expression Vector 10 μg (E5269) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
• pFLAG-CTS-BAP Control Plasmid 1 μg (P7707) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.

General description

The pFLAG-CTS Expression Vector is a 5.3 kb E. coli expression vector used for cytoplasmic expression of a properly inserted open reading frame as a C-terminal FLAG® fusion protein. The FLAG epitope is a small, hydrophilic 8 amino acid tag (DYKDDDDK) that provides for sensitive detection and high quality purification using ANTI-FLAG products.

C-terminal FLAG fusion proteins may be purified using Monoclonal ANTI-FLAG M2, Catalog Number F3165, and ANTI-FLAG M2 Affinity Gel, Catalog Number A2220.

The pFLAG-CTS-BAP Control Plasmid is a 6.7 kb E. coli plasmid used for efficient and controlled periplasmic expression of C-terminal FLAG-BAP fusion protein.

Vector Maps and Sequences

Principle

The promoter-regulatory region of the strong tac promoter (a hybrid of the trp and lac promoters from E.coli) drives transcription of ORF-FLAG fusion constructs. Control of transcription is regulated by the presence of the lacO sequences and inclusion of the lac repressor gene (lacI) on the plasmid.

Legal Information

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

pFLAG-CTC is a trademark of Sigma-Aldrich Co. LLC

pFLAG-CTS is a trademark of Sigma-Aldrich Co. LLC

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

pSF-FLAG® Vectors from Oxford Genetics

The classic FLAG and 3xFLAG expression vectors have been converted to SnapFast™ Expression Vectors – combining two highly functional systems into one product. Additional information about the SnapFas...
Keywords: Flow cytometry, Gene expression, Immunocytochemistry, Immunoprecipitation, Purification, Western blot

Peer-Reviewed Papers
15

References

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