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ED4SS Sigma-Aldrich

Ethylenediaminetetraacetic acid tetrasodium salt dihydrate

99.0-102.0% (titration)

Synonym: EDTA tetrasodium salt, Edathamil, Tetrasodium ethylenediaminetetraacetate dihydrate

  • CAS Number 10378-23-1

  • Linear Formula (NaOOCCH2)2NCH2CH2N(CH2COONa)2 · 2H2O

  • Molecular Weight 416.20

  •  Beilstein/REAXYS Number 3861753

  •  EC Number 200-573-9

  •  MDL number MFCD00150026

  •  PubChem Substance ID 24894711

  •  NACRES NA.77



Related Categories Buffers, Calcium Signaling Research Tools, Cell Biology, Cell Signaling and Neuroscience, Core Bioreagents,
Quality Level   200
assay   99.0-102.0% (titration)
solubility   H2O: soluble, clear, colorless
SMILES string   O.O.[Na+].[Na+].[Na+].[Na+].[O-]C(=O)CN(CCN(CC([O-])=O)CC([O-])=O)CC([O-])=O
InChI   1S/C10H16N2O8.4Na.2H2O/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;;;;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;;;;2*1H2/q;4*+1;;/p-4



1 kg in poly bottle

100, 500 g in poly bottle

2.5 kg in poly drum


Used to eliminate enzyme inhibition by traces of heavy metals, and to inhibit enzymes that require divalent cations as cofactors.

Safety & Documentation

Safety Information

Signal word 
Hazard statements 
Target organs 
Respiratory Tract
Personal Protective Equipment 
NONH for all modes of transport


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Single- & multi-channel pipettes from BRAND
Protocols & Articles


Enzymatic Assay of Cathepsin B

This procedure applies to all products that have a specification for Cathepsin B activity, such as Sigma-Aldrich Product Numbers C0150 and C8571, determined by the liberation of 7-amino-4-methylcouma...
Keywords: Enzyme activity, Enzymology

Enzymatic Assay of Ficin (EC

The spectrophotometric stop rate detemination (A280, Light path = 1 cm) is based on the following reaction:

Enzymatic Assay of α-Glucosidase by the Modified Boehenger Procedure (EC

To standardize a procedure for the enzymatic assay of α-glucosidase by the modified Boehenger procedure at Sigma-Aldrich St. Louis.
Keywords: Enzyme activity, Extinction coefficient

Enzymatic assay of lipase type XIII from Pseudomonas sp. and Pseudomonas cepacia using a coupled enzyme system of glycerol kinase and glycerophosphate oxidase (EC

To standardize a procedure for the enzymatic assay of Lipase Type XIII from Pseudomonas sp., Product No. L9518 , and Pseudomonas cepacia, using a coupled enzyme system of glycerol kinase and glycerop...
Keywords: Centrifugation, Extinction coefficient, High performance liquid chromatography, Size-exclusion chromatography

Immunohistochemistry Protocol

Immunohistochemical staining is a valuable tool for detecting specific antigens in tissues. In order to perform the standard staining procedure, first the tissue section has to be deparaffinized and ...
Keywords: Digestions, Immunohistochemistry, Solvents

Recycling Micro-Assay of β-NAD and β-NADH

1. OBJECTIVE To standardize a procedure for the recycling micro-assay of β-NAD and β-NADH at Sigma-Aldrich Saint Louis.
Keywords: Sonication

Peer-Reviewed Papers


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03695 Ethylenediaminetetraacetic acid tetrasodium salt dihydrate, purum, ≥98.0% (KT)
E6511 Ethylenediaminetetraacetic acid tetrasodium salt dihydrate, BioReagent, suitable for cell culture, 98.5-102.0%

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