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EDEGLY Sigma-Aldrich

Enzymatic Protein Deglycosylation Kit

  •  NACRES NA.32




Enzymatic Protein Deglycosylation Kit has been used for the deglycosylation of various enzymes.

Kit component only


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PNGase F 1 mL/vial    
O-Glycosidase 20 μL    
α-2(3,6,8,9)-Neuraminidase 20 μL    
Fetuin Control .5 mg    
5× Reaction Buffer .2 mL    
Denaturation Solution .1 mL    
Triton X-100 .1 mL    
β-1→4-Galactosidase, positionally specific 20 μL    
β-N-Acetylglucosaminidase 20 μL    
Safety & Documentation

Safety Information

UN 3316 9
Protocols & Articles


Deglycosylation Kits

GlycoProfile™ I Enzymatic In-gel N-Deglycosylation Kit GlycoProfile™ II Enzymatic In-solution N-Deglycosylation Kit Enzymatic Protein Deglycosylation Kit Native Protein Deglycosylation Kit
Glycobiology Analysis Manual, 2nd Edition
Keywords: Degradations, Digestions, Glycosylations, High performance liquid chromatography, Mass spectrometry, PAGE, Proteomics

GlycoProfile™ Labeling Kits: Useful Fluorescent Dyes for Enhanced Glycan Analysis

Glycan analysis has become an increasingly critical aspect of glycomics and proteomics, as the role of glycoproteins in cell signaling, cell adhesion, immune response, and disease states is emerging ...
BioFiles 2007, 2.1, 14.
Keywords: Adhesion, Aminations, Atomic absorption spectroscopy, Capillary electrophoresis, Cell signaling, Chromatography, Coupling reactions, Filtration, Glycosylations, High performance liquid chromatography, Ion Exchange, Mass spectrometry, Normal-phase chromatography, Phase transitions, Proteomics, Reductions, Reductive aminations, Separation

Glycoprotein Deglycosylation

Aparagine-linked (N-linked) and serine/threonine-linked (O-linked) oligosaccharides are major structural components of many eukaryotic proteins. They perform critical biological functions in protein ...
BioFiles 2007, 2.1, 7.
Keywords: Degradations, Dialysis, Digestions, Filtration, Glycosylations, High performance liquid chromatography, Inflammation, Ligands, Mass spectrometry, Methods, PAGE, Proteomics, Substitutions, Type

N-Linked Glycan Strategies

Use of the endoglycosidic enzyme PNGase F (N-Glycosidase F) is the most effective method of removing virtually all N-linked oligosaccharides from glycoproteins. PNGase F cleaves all asparagine-linked...
Glycobiology Analysis Manual, 2nd Edition
Keywords: Applications, Detergents, Digestions, Mass spectrometry, Methods, Nucleic acid denaturation, Proteomics

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