Articles
Accelerated Osteocyte Differentiation of Human Mesenchymal Stem Cells in a Novel Xeno-free and Serum-free Osteogenesis Differentiation Media
Mesenchymal stem cells (MSCs) have the capacity for multi-lineage differentiation, giving rise to a variety of mesenchymal phenotypes such as osteoblasts (bone), adipocytes (fat), and chondrocytes (c...
Anna Abai, Nick Asbrock and Vi Chu1
1 MilliporeSigma, Bioscience Division, Temecula, CA, USA
Keywords: Cell culture, Clinical, Drug discovery, Gene expression, Immunostaining
Derivation of Functional Oligodendrocyte Progenitor Cells (OPCs) from Human Neural Stem Cell Lines
Introduction Methods Human Neural Stem Cell Culture Oligodendrocyte Differentiation and Maturation Oligodendrocyte Progenitor Cell Characterization In Vitro Myelination Assay Results Conc...
Christine Chen, Michael Moeller, Anna Abai, Nick Asbrock and Vi Chu1
1 MilliporeSigma, Bioscience Division, Temecula, CA, USA
Keywords: Adhesion, Atomic absorption spectroscopy, Cell culture, Cell proliferation, Central Nervous System, Clinical, Culture media, Diseases, Gene expression, Growth factors, Hormones, Neurodegenerative Diseases, Pharmaceutical, Phase transitions, RNA immunoprecipitation, Transfection
Fibroblast Growth Factors for Cell Culture
Fibroblast Growth Factors (FGFs) are potent regulators of cell proliferation, differentiation. They are critically important in normal development, tissue maintenance, wound repair and angiogenesis i...
Keywords: Angiogenesis, Cancer, Cardiovascular, Cell culture, Cell proliferation, Diabetes, Diseases, Gene expression, Growth factors, Ligands
Growth Factors in Stem Cell Biology
Stem cell biology researchers use suitable growth factors to trigger proliferation, differentiation and/or migration of stem cells. Embryonic pluripotent stem cells can differentiate into three germ ...
Keywords: Angiogenesis, Cell biology, Cell culture, Growth factors, Stem cell biology
Naive Pluripotent Stem Cell Culture in an Inhibitor Based 2i/LIF Containing Serum-Free and Feeder-Free Stem Cell Media
Naive pluripotent stem cells are located within the epiblast of mature blastocysts. These primitive “ground-state” cells may be cultured in vitro using specialized media and small molecule inhibitors...
Gabi Tremml, Ming Li and Vi Chu1*,
1MilliporeSigma, Bioscience Division, Temecula, CA, USA
Keywords: Cell culture, Gene expression, Immunocytochemistry, Microscopy, Polymerase chain reaction, Polymerase chain reaction - quantitative, Transcription
Neural Stem Cell FAQs
The use of neural stem cells (NSCs) in biomedical research is becoming increasingly popular, resulting in breakthrough studies rejected the longstanding belief that neuronal tissue is incapable of re...
Keywords: Cell culture, Central Nervous System, Centrifugation, Clinical, Diffusion, Digestions, Diseases, Gene expression, Immunocytochemistry, Neurodegenerative Diseases, Parkinson Disease
PLTMax® Human Platelet Lysate (hPL): A Superior Serum-Free Media Supplement for Robust Mesenchymal Stem Cell Expansion
Introduction Methods Human MSC Expansion in Human Platelet Lysate Human MSC Differentiation FACS Mesenchymal Stem Cell Marker Analysis Results Conclusions Materials
Aletta Schnitzler, Manjula Aysola, Anjali Verma, Tristan Lawson, Susan Rigby, Megan Pease, Sandhya Punreddy, Julie Murrell, Martha Rook
Stem Cell Bioprocessing Group
Keywords: Cell culture, Cell disruption, Clinical, Culture media, Flow cytometry, Gene expression, Growth factors, Polymerase chain reaction, Stem cell biology
Predictive Models for Neuroscience Research
For neuroscientists, the quest for truly predictive neural models has its challenges given the brain is a very complex organ comprised of many diverse cell types, complex cell-tocell interactions and...
Keywords: Apoptosis, Cell culture, Cell proliferation, Central Nervous System, Deposition, Diseases, Dopamine agents, Drug discovery, Environmental, Filtration, Gene expression, Genetic, Growth factors, Neuroscience, Reductions, Schizophrenia, Sequencing, Sterilizations, Transfection
Mesenchymal Stem Cell Culture Protocols | MSC Culture Media
Introduction Methods Isolation of Mesenchymal Stem Cells Expansion of Human Mesenchymal Stem Cells Preparation of Coated Plates Thawing of Mesenchymal Stem Cells Expansion of Mesenchy...
Keywords: Cell disruption, Clinical, Culture media, Gene expression, Immunocytochemistry, Stem cell biology
Neural Stem Cell Culture Protocols
Introduction Methods Isolation of Neural Stem Cells Isolation of NSCs from Neural Tissue Differentiation of Human iPSCs into NSCs Characterization of Neural Stem Cells ...
Keywords: AGE, Adhesion, Apoptosis, Cell culture, Central Nervous System, Cryopreservation, Culture media, Diseases, Growth factors, High performance liquid chromatography, Phase transitions, Phosphorylations, Purification
Cell Culture Troubleshooting Guide
Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions