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F5542 Sigma-Aldrich

Fibroblast Growth Factor-Acidic human

FGF-Acidic, recombinant, expressed in E. coli, suitable for cell culture

Synonym: FGF-1, aFGF

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Properties

Related Categories Angiogenesis, Angiogenic Factors, Cancer Research, Cell Biology, Cell Culture,
Quality Level   200
biological source   human
recombinant   expressed in E. coli
assay   ≥97% (SDS-PAGE)
form   lyophilized powder
potency   0.287 ng/mL
quality   endotoxin tested
mol wt   15.5 kDa
packaging   pkg of 25 μg
storage condition   avoid repeated freeze/thaw cycles
application(s)   cell culture | mammalian: suitable
impurities   ≤1.0 EU/mg (LAL test)
UniProt accession no.   P05230
storage temp.   −20°C
Gene Information   human ... FGF1(2246)

Description

Biochem/physiol Actions

A potent mitogenic agent for a wide variety of mesoderm-derived cells including BALB/c 3T3 fibroblasts, capillary and endocardial endothelial cells, myoblasts, vascular smooth muscle cells, mesothelial cells, glial and astroglial cells, and adrenal cortex

Physical form

The product is lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 1 M NaCl, 5 mM DTT containing 50 μg of bovine serum albumin per 1 μg of cytokine.

Analysis Note

The proliferative activity is tested in culture using quiescent NR6R-3T3 fibroblasts.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Fibroblast Growth Factors for Cell Culture

Fibroblast Growth Factors (FGFs) are potent regulators of cell proliferation, differentiation. They are critically important in normal development, tissue maintenance, wound repair and angiogenesis i...
Keywords: Angiogenesis, Cancer, Cardiovascular, Cell culture, Cell proliferation, Diabetes, Diseases, Gene expression, Growth factors, Ligands

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Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

Peer-Reviewed Papers
15

References

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