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F5542 Sigma-Aldrich

Fibroblast Growth Factor-Acidic human

FGF-Acidic, recombinant, expressed in E. coli, suitable for cell culture

Synonym: FGF-1, aFGF

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Properties

Related Categories Angiogenesis, Angiogenic Factors, Cancer Research, Cell Biology, Cell Culture,
Quality Level   200
biological source   human
recombinant   expressed in E. coli
assay   ≥97% (SDS-PAGE)
form   lyophilized powder
potency   0.287 ng/mL
quality   endotoxin tested
mol wt   15.5 kDa
packaging   pkg of 25 μg
storage condition   avoid repeated freeze/thaw cycles
application(s)   cell culture | mammalian: suitable
impurities   ≤1.0 EU/mg (LAL test)
UniProt accession no.   P05230
storage temp.   −20°C
Gene Information   human ... FGF1(2246)

Description

General description

Fibroblast Growth Factor-Acidic (FGF1) belongs to the FGF family and comprises a nuclear export sequence (NES). FGF1 gene is mapped to human chromosome 5q31.3. FGF1 is highly expressed in the central and peripheral nervous system.

Application

Fibroblast Growth Factor-Acidic human has been used:
• as a component of growth factor cocktail for treating neural stem cell (NSC) transplants
• as a component of Human Neural Stem Cell (HNSC) proliferation medium for Neural Tissue Spheres (NTS) propagation
• as a growth factor component in fibrin matrix to support embryonic day 14 (E14) cells graft survival

Biochem/physiol Actions

Fibroblast Growth Factor-Acidic (FGF1) is a potent mitogenic agent associated with a variety of tissue origins including liver, vasculature, and skin. These include for a wide variety of mesoderm-derived cells including BALB/c 3T3 fibroblasts, capillary and endocardial endothelial cells, myoblasts, vascular smooth muscle cells, mesothelial cells, glial and astroglial cells, and adrenal cortex. FGF1 participates in glucose homeostasis, adipose tissue remodeling and is a key transducer of the nuclear receptor, peroxisome proliferator-activated receptor γ (PPARγ). FGF1 is a neuronal cell neurotrophic factor and binds to most of the isoforms of the FGF receptor (FGFR) isoforms. Along with FGF2, FGF1 plays a key role in angiogenesis and favors blood vessel formation in chronic inflammatory diseases and cancer. The FGF1 mutant R50E leads to suppression of its angiogenic potential making it a potential anti-angiogenesis agent.

Physical form

The product is lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 1 M NaCl, 5 mM DTT containing 50 μg of bovine serum albumin per 1 μg of cytokine.

Analysis Note

The proliferative activity is tested in culture using quiescent NR6R-3T3 fibroblasts.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Fibroblast Growth Factors for Cell Culture

Fibroblast Growth Factors (FGFs) are potent regulators of cell proliferation, differentiation. They are critically important in normal development, tissue maintenance, wound repair and angiogenesis i...
Keywords: Angiogenesis, Cancer, Cardiovascular, Cell culture, Cell proliferation, Diabetes, Diseases, Gene expression, Growth factors, Ligands

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Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

Peer-Reviewed Papers
15

References

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