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G1512 Sigma-Aldrich

β-Glucuronidase from Helix pomatia

Type H-5, lyophilized powder, ≥400,000 units/g solid

Synonym: β-D-Glucuronide glucuronosohydrolase

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Description

General description

β-Glucuronidase Type H-2 from Helix pomatia is a crude solution of enzymes derived from the Roman snail. Many β-glucuronidases derived from mollusks also contain sulfatase activity.

Application

β-Glucuronidase from Helix pomatia has been used:
• in the enzymatic treatment of urine samples for liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) analysis
• in the quercetin extraction procedure from pig or rat tissues for high performance liquid chromatography (HPLC) analysis
• in the enzymatic deconjugation step in urine and plasma sample preparation for gas chromatography-mass spectrometry analysis (GC-MS)

β-glucuronidase was used to hydrolyze conjugated glucuronide and sulfate metabolites.

New Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine

Packaging

100000, 500000 units in poly bottle

Biochem/physiol Actions

β-glucuronidase (β-GIc) is an exoglycosidase that catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption.

Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis

Quality

Many β-glucuronidases derived from mollusks also contain sulfatase activity.

Linkage

A further purification of G0876 by gel permeation chromatography.

Unit Definition

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 5.0 (30 min assay).

Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C.

Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
LZ8990000

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Evaluation of Different Enzymes on Hydrolysis Efficiencies of Glucuronide Drug Metabolites in Urine

β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS. Here we evaluate the hydrolysis efficiency of β-gluc...
Jim Blasberg
Harkewal Singh, Kevin Ray Sigma-Aldrich, St. Louis, MO
Keywords: High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolites

UHPLC/MS of Drugs and Metabolites in Urine following Enzymatic Hydrolysis

β-D-Glucuronide glucuronosohydrolase enzymes, commonly shortened to β-glucuronidase, play an important role in the analysis of biological fluids for the presence of metabolites for drug screening and...
Craig Aurand, Manager; Kristen Brown, Pennsylvania State University
Reporter US Volume 33.2
Keywords: Carbohydrate metabolism, Derivatizations, Digestions, Enzyme activity, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolism, Metabolites, Sample preparations, Separation, Solid phase extractions, Solvents

Protocols

Enzymatic Assay of ß-Glucuronidase (EC 3.2.1.31) from Helix Pomatia and Bovine Liver

This procedure applies to all b-Glucuronidase products that are derived from Helix Pomatia and Bovine Liver.
Keywords: Enzymology

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Peer-Reviewed Papers
15

References

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