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G1777 Sigma-Aldrich

Glial Cell Line-derived Neurotrophic Factor human

recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture, ≥98% (SDS-PAGE)

Synonym: ATF, GDNF

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Properties

Related Categories Cell Biology, Cell Culture, Cell Signaling and Neuroscience, Cytokines and Growth Factors, Cytokines, Growth Factors and Hormones,
biological source   human
recombinant   expressed in E. coli
assay   ≥98% (SDS-PAGE)
form   lyophilized powder
mol wt   ~30 kDa
packaging   pkg of 10 μg
storage condition   avoid repeated freeze/thaw cycles
application(s)   cell culture | mammalian: suitable
impurities   endotoxin, tested
color   white
UniProt accession no.   P39905
storage temp.   −20°C
Gene Information   human ... GDNF(2668)

Description

General description

Glial cell line-derived neurotrophic factor (GDNF) human gene is located on human chromosome 5p13.2.

Application

Glial cell line-derived neurotrophic factor human (GDNF) has been used as a component in the neurobasal medium for neural differentiation. It has also been used for self-renewal, expansion and differentiation of spermatogonial stem cells (SSCs).

Biochem/physiol Actions

Glial Cell Line-Derived Neurotrophic Factor is a member of the cysteine-knot superfamily of growth factors that assume stable dimeric protein structures. GDNF is founding member of the GDNF family of ligands, which to date include GDNF, neurturin (NTN), persephin (PSP) and artemin (ART). GDNF is a glycosylated disulfide-linked homodimeric protein of ~15 kDa. Mature rat and human GDNF share ~93% sequence homology, with strong species cross-reactivity. GDNF signals through a multicomponent receptor system, composed of a RET and one of the four GFRα (α1-α4) receptors. GDNF specifically promotes dopamine uptake and survival and morphological differentiation of midbrain neurons. Using the Parkinson′s disease mouse model, GDNF has been shown to improve conditions such as bradykinesia, rigidity, and postural instability. GDNF promotes survival of various neuronal cells in central and peripheral nervous systems and different stages of development, including motoneurons, midbrain dopaminergic neurons, Purkinje cells and sympathetic neurons. Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes and skeletal muscle cells. In addition, exogenously applied GDNF has been shown to rescue damaged facial motor neurons in vivo.

Glial cell line-derived neurotrophic factor human (GDNF) acts as a morphogen in kidney development and modulates spermatogonial differentiation. Mutations in this gene may be associated with Hirschsprung′s disease, Tourette syndrome (TS) and attention deficit/ hyperactivity disorder (ADHD). It is used to treat Parkinson′s disease.

Physical form

Lyophilized from a 0.2 μm filtered solution of 10 mM sodium citrate and 150 mM sodium chloride containing 0.5 mg bovine serum albumin.

Analysis Note

The biological activity of GDNF is determined by the dose-dependent dopamine uptake by rat ventral mesencephalic cultures.

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Growth Factors in Stem Cell Biology

Stem cell biology researchers use suitable growth factors to trigger proliferation, differentiation and/or migration of stem cells. Embryonic pluripotent stem cells can differentiate into three germ ...
Keywords: Angiogenesis, Cell biology, Cell culture, Growth factors, Stem cell biology

Predictive Models for Neuroscience Research

For neuroscientists, the quest for truly predictive neural models has its challenges given the brain is a very complex organ comprised of many diverse cell types, complex cell-tocell interactions and...
Keywords: Apoptosis, Cell culture, Cell proliferation, Central Nervous System, Deposition, Diseases, Dopamine agents, Drug discovery, Environmental, Filtration, Gene expression, Genetic, Growth factors, Neuroscience, Reductions, Schizophrenia, Sequencing, Sterilizations, Transfection

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Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

Peer-Reviewed Papers
15

References

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