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G5758 Sigma-Aldrich

Glucose-6-Phosphatase from rabbit liver

≥0.05 units/mg protein, Protein ~30 % (balance mostly sucrose)

Synonym: D-Glucose-6-Phosphate Phosphohydrolase

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Properties

Related Categories 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Biochemicals and Reagents, Carbohydrate Metabolism, Cell Biology,
Quality Level   200
form   powder with sucrose
specific activity   ≥0.05 units/mg protein
composition   Protein, ~30% (balance mostly sucrose)
storage temp.   −20°C

Description

General description

Glucose-6-phosphatase is a multicomponent protein complex comprising of glucose-6-phosphate transporter (G6PT) and a catalytic unit (G6PC). Liver and kidney cells show predominant expression of glucose-6-phosphatase.

Application

Glucose-6-Phosphatase from rabbit liver has been used in glucose 6-phosphatase inhibitory assay of crude C. anisata leaf extracts and methanolic leaf and bark extracts of Dolichandrone atrovirens.

Glucose-6-Phosphatase was used to study the effects of Clausena anisata (Wild hook leaf) extracts on selected diabetes-related metabolizing enzymes.

Packaging

5, 25 units in poly bottle

Biochem/physiol Actions

Glucose-6-Phosphatase is crucial for regulating glucose homeostasis during starvation. Deficiency of glucose-6-phosphatase is implicated in glycogen storage disease type I (GSDI).

Glucose-6-phosphatase catalyzes the conversion of glucose-6-phosphate into glucose as the last step of gluconeogenesis.

Other Notes

It has been reported that glucose-6-phosphatase, inorganic pyrophosphatase, and pyrophosphate glucose phosphotransferase are associated with a single protein that catalyzes three different reactions:
a) glucose 6-phosphate + H2O → glucose + Pi
b) PPi + H2O → 2 Pi
c) glucose + PPi or nucleoside-DP or -TP → glucose 6-phosphate + Pi or nucleoside-MP.

Quality

Crude microsomal preparation

Unit Definition

One unit will release 1.0 μmole of inorganic phosphate from glucose 6-phosphate per min at pH 6.5 at 37 °C.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Insulin Signaling and Energy Homeostasis

Glucose metabolism is regulated by the opposing actions of insulin and glucagon. Insulin is released from pancreatic ß cells in response to high blood glucose levels and regulates glucose metabolism ...
Linda Stephenson, Ph.D.
Biofiles v6 n4, 2011
Keywords: Apoptosis, Biological processes, Carboxylations, Catalog, Diabetes, Gene expression, Gluconeogenesis, Glycolysis, Hormones, Metabolism, Metabolites, Transcription, Transduction, Type

Protocols

Enzymatic Activity of Glucose-6-Phosphatase [EC 3.1.3.9]

Replaces enzyme assay procedure SSGLUC02. Document number and formatting changed to reflect current numbering and formatting guidelines. The procedure was modified to include a standard curve that re...
Keywords: Enzyme activity, Enzymology

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Enzymes & Proteins

Committed to developing and providing breakthrough problem-solving reagents and solutions, explore our comprehensive offering of enzymes and protein reagents and kits with the confidence that you wil...
Keywords: Cell signaling, Diagnostic, Enzyme-linked immunosorbent assay, Immunohistochemistry, Metabolism, Molecular biology, Purification, Western blot

Peer-Reviewed Papers
15

References

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