G7126 Sigma-Aldrich


ReagentPlus®, ≥99% (HPLC)

Synonym: Aminoacetic acid, Aminoethanoic acid, Glycocoll

  • CAS Number 56-40-6

  • Linear Formula NH2CH2COOH

  • Molecular Weight 75.07

  •  Beilstein/REAXYS Number 635782

  •  EC Number 200-272-2

  •  MDL number MFCD00008131

  •  eCl@ss 32160406

  •  PubChem Substance ID 24895290

  •  NACRES NA.26



Related Categories Allium cepa (Onion), Allium sativum (Garlic), Amino Acid, Amino Acids, Biochemicals and Reagents,
Quality Level   200
product line   ReagentPlus®
assay   ≥99% (HPLC)
form   powder
application(s)   western blot: suitable
impurities   ≤0.01% Ammonia
loss   ≤0.2% loss on drying
color   white to off-white
pKa (25 °C)   (1) 2.35, (2) 9.60
mp   240 °C (dec.) (lit.)
solubility   H2O: 200 mg/mL, clear, colorless to faintly yellow
SMILES string   NCC(O)=O
InChI   1S/C2H5NO2/c3-1-2(4)5/h1,3H2,(H,4,5)
Gene Information   rat ... Grin2a(24409)


General description

Glycine is a non-chiral amino acid. Glycine is a molecule with pI of 6.7, which is similar to the pH of stacking region in polyacrylamide gels. It has advantage of low mobility, hydrophobicity and it does not associate with proteins.


• Glycine has been added in the transfer buffer during western blotting procedure.
• It has been used in the solution prepared for dissolving formazan crystals in the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyltetrazolium bromide) cytotoxicity assay.
• It has been used for terminating the rat basophilic leukemia (RBL) assay.


1 kg in poly bottle

10 mg in glass bottle

100, 500 g in poly bottle

5, 25 kg in poly drum

Biochem/physiol Actions

Glycine is an inhibitory neurotransmitter in the central nervous system and works as an allosteric regulator of NMDA (N-methyl-D-aspartate) receptors. It is involved in processing of motor and sensory data, thereby regulating movement, vision and audition.

Inhibitory neurotransmitter in spinal cord, allosteric regulator of NMDA receptors.

Legal Information

ReagentPlus is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles


HPLC Analysis of Mouse Monoclonal Antibodies (MAb) on TSKgel® Ether-5PW

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Mouse Monoclonal Antibodies (MAb) on TSKgel® Ether-5PW
Keywords: Chromatography, High performance liquid chromatography

MSMLS Online Plate Map

Metabolite descriptors included with the software download upon purchase of the product include: Name, Parent CID, molecular formula, molecular weight, CAS, ChEBI, HMDB ID, PubChem Compound and Subst...
Keywords: Mass spectrometry, Metabolites

Nucleotide Synthesis in Cancer Cells

Neoplastic cells are highly dependent on the de novo synthesis of nucleotides to maintain sufficient pools to support DNA replication and the production of RNA. The metabolic pathways supporting nucl...
BioFiles v7 n4
Keywords: Cancer, Catalysis, Citric Acid Cycle, DNA replication, Gene expression, Glycolysis, Metabolic Pathways, Metabolism, Metabolites, Nucleotide Synthesis, Pentose phosphate pathway, Pyrimidine synthesis


Enzymatic Assay and ATP Sensitivity Test of Luciferase

To standardize a procedure to determine the enzymatic activity of Luciferase and/or to determine the ATP detection limit of Luciferase.
Keywords: Biochemistry, Enzyme activity, Enzymology, Size-exclusion chromatography

Enzymatic Assay of 5’-Nucleotidase

This procedure applies to all products that have a specification for 5’-Nucleotidase. This procedure is NOT to be used to assay 5’-Nucleotidase from bovine liver, Sigma-Aldrich Product Number N2779, ...
Keywords: Chromatography, Enzyme activity

Enzymatic Assay of Choloylglycine Hydrolase

To standardize a procedure for the determination of the enzymatic assay of choloylglycine hydrolase.
Keywords: Enzyme activity

Enzymatic Assay of Phytase (EC

Derived from procedure SSPHYT02. Includes template updates to current SOP specifications and incorporation of notes into the procedure. Refer to SOP-DEK-ENZ18.
Keywords: Biochemistry

Enzymatic Assay of ß-Glucuronidase (EC from Helix Pomatia and Bovine Liver

This procedure applies to all b-Glucuronidase products that are derived from Helix Pomatia and Bovine Liver.
Keywords: Enzymology

Enzymatic Assay of β-Glucuronidase (EC from E. coli

The scope of this procedure is all products from E. coli that have a specification for β-Glucuronidase activity.
Keywords: Enzymology

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Amino Acids Reference Charts

Amino acids are the compounds or building blocks that make up peptides and proteins. Each amino acid is structured from an amino group and a carboxyl group bound to a tetrahedral carbon. This carbon ...
Keywords: Building blocks, Gene expression, Mass spectrometry

BioUltra Biological Buffers

A buffer, as defined by Van Slyke [1], is "a substance which by its presence in solution increases the amount of acid or alkali that must be added to cause unit change in pH". Buffers are thus very i...
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Peer-Reviewed Papers


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50046 Glycine, BioUltra, for molecular biology, ≥99.0% (NT)

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