• USA Home
  • GE17-0403-01 - HiTrap® Protein A High Performance

EMAIL THIS PAGE TO A FRIEND
GE17-0403-01

HiTrap® Protein A High Performance

GE Healthcare, 17-0403-01, pack of 1 × 5 mL

  •  NACRES NA.56

Purchase

Properties

Related Categories Analytical/Chromatography, GE Healthcare Life Sciences Protein Sample Preparation Formats, HiTrap & PD-10 Cartridges, HiTrap System/syringe, Molecular Biology,
shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging   pack of 1 × 5 mL
mfr. no.   GE Healthcare, 17-0403-01
parameter   flow rate
  <20 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
  42 psi
bed size   16 mm × 25 mm
bed volume   5 mL
column I.D.   16 mm
matrix   highly cross-linked 6% agarose
avg. part. size   34 μm
cleaning   2 - 10 (pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)
working range   3 - 9
storage temp.   2-8°C

Description

General description

HiTrap® Protein A HP is a prepacked Protein A Sepharose® High Performance column for preparative purification of monoclonal and polyclonal antibodies from ascites, serum, and cell culture supernatants.

HiTrap® Protein A HP is a prepacked ready to use, column for preparative purification of monoclonal and polyclonal antibodies. The special design of the column, together with the medium provide fast, simple and easy separations in a convenient format.

Features and Benefits

• High binding capacitiy of 20 mg IgG/mL medium and fast kinetics.
• 34 μm bead size for high-performance, high-resolution purifications.
• Designed for use with syringe, pump, or chromatography system such as AKTA design or FPLC System.
• Stable over pH 3 to 9 for use, and 2 to 10 for cleaning.
• Robust, easy-to-use method.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.gelifesciences.com.

Legal Information

FPLC is a trademark of Cytiva

HiTrap is a registered trademark of Cytiva

Sepharose is a registered trademark of Cytiva

Safety & Documentation

Safety Information

RIDADR 
UN1170 - class 3 - PG 3 - Ethanol, solution

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Articles

General Instructions for Affinity Purification Using HiTrap® Columns

Appendix 4, Extracted from Affinity Chromatography Vol. 1: Antibodies, GE Healthcare, 2016  
Keywords: Affinity chromatography, Buffers, Chromatography, PAGE

Performing a Purification of IgG Antibodies with HiTrap Protein A HP columns

HiTrap® Protein A HP are 1 ml and 5 ml ready-to-use columns prepacked with Protein A Sepharose® High Performance (Figure 3.17). The columns are used for convenient purification of antibodies from cell...
Keywords: Affinity chromatography, Cell culture, Chromatography, Immunodiffusion, PAGE, Sample preparations

Performing a Purification of IgG Antibodies with HiTrap® Protein G HP Columns

HiTrap® Protein G HP (Figure 3.10) is a convenient, ready-to-use column prepacked with Protein G Sepharose® High Performance. The columns are available in 1 ml and 5 ml sizes. In common with all HiTr...
Keywords: Affinity chromatography, Buffers, Cell culture, Chromatography, Immunodiffusion, Ligands, PAGE, Sample preparations

Performing a Purification of IgM Antibodies with HiTrap™ IgM Purification HP

HiTrap™ IgM Purification HP 1 ml columns are prepacked with a thiophilic adsorption medium (2-mercaptopyridine coupled to Sepharose® High Performance). The binding capacity of HiTrap™ IgM Purification ...
Keywords: Adsorption, Affinity chromatography, Cell culture, Chromatography, Enzyme-linked immunosorbent assay, Ligands, PAGE, Precipitation, Purification, Sample preparations

Selection Guide for Polymeric Resins in Chromatography & Purification

Sigma-Aldrich carries a vast array of resins, from leading manufacturers; such as, Dow Chemical, Rohm & Haas, Mitsubishi, and Lanxess. Our product line includes adsorbent, ion-exchange (anion / catio...
Keywords: Adsorption, Antibiotics, Combinatorial synthesis, Detergents, Fermentation, Filtration, Hormones, Ion Exchange, Ion exchange resins, Pharmaceutical, Purification, Separation

Protocols

Affinity Chromatography Troubleshooting

This section focuses on practical problems that may occur when running a chromatography column. The diagrams below give an indication of how a chromatogram may deviate from the ideal during affnity p...
Keywords: Adsorption, Affinity chromatography, Buffers, Chromatography, Detergents, Digestions, Fractionation, Ligands, PAGE, Precipitation, Purification, Sample preparations, Separation, Solvents

Buffer Exchange and Desalting for Affinity Chromatography

Dialysis is frequently mentioned in the literature as a technique to remove salt or other small molecules and to exchange the buffer composition of a sample. However, dialysis is generally a very slo...
Keywords: Absorption, Affinity chromatography, Buffers, Chromatography, Dialysis, Separation

Column Packing and Preparation for Affinity Chromatography

Appendix 3,  Extracted from Affinity Chromatography Principles and Methods, GE Healthcare, 2007
Keywords: Affinity chromatography, Antimicrobials, Buffers, Chromatography, Separation

Converting From Linear Fow (cm/hour) to Volumetric Flow Rates (ml/min) and Vice Versa

Appendix 4 extracted from Affinity Chromatography Principles and Methods, GE Healthcare, 2007
Keywords: Affinity chromatography, Chromatography

Performing a Separation with GE Healthcare Products Based on Protein A

Protein A is derived from a strain of Staphylococcus aureus and contains five regions that bind to the Fc region of IgG. As an affinity ligand, protein A is coupled to Sepharose so that these regions a...
Keywords: Affinity chromatography, Buffers, Cell culture, Chromatography, Ligands, PAGE, Purification

Sample Preparation for Affinity Chromatography

Appendix 1 extracted from Affinity Chromatography Principles and Methods, GE Healthcare, 2007
Keywords: Adsorption, Affinity chromatography, Buffers, Centrifugation, Chromatography, Detergents, Filtration, Nucleic acid denaturation, PAGE, Precipitation, Sample preparations

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?