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GE17-0404-01

HiTrap® Protein G High Performance

GE Healthcare, 17-0404-01, pack of 5 × 1 mL

  •  NACRES NA.56

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Properties

Related Categories Analytical/Chromatography, GE Healthcare Life Sciences Protein Sample Preparation Formats, HiTrap & PD-10 Cartridges, HiTrap System/syringe, Molecular Biology,
shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging   pack of 5 × 1 mL
mfr. no.   GE Healthcare, 17-0404-01
parameter   <4 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
  42 psi
bed size   7 mm × 25 mm
bed volume   1 mL
column I.D.   7 mm
avg. part. size   34 μm
cleaning   2 - 9 (pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)
working range   3 - 9 (pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)
capacity   >1 mg binding capacity (lgG/column)(The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
  25 mg binding capacity(human IgG/column)
storage temp.   2-8°C

Description

General description

HiTrap® Protein G HP columns are prepacked with Protein G Sepharose® High Performance for fast, easy separations of IgG from different species and subclasses.

Features and Benefits

• High binding capacity of 25 mg IgG/mL medium and fast binding kinetics.
• 34 μm bead size for high performance, high-resolution purifications.
• Binds broad range of different species and subclasses of IgG.
• Simple and proven method giving reproducible results.
• Designed for use with syringe, peristalic pump, or chromatography system.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.gelifesciences.com.

Legal Information

HiTrap is a registered trademark of Cytiva

Sepharose is a registered trademark of Cytiva

Safety & Documentation

Safety Information

RIDADR 
UN1170 - class 3 - PG 3 - Ethanol, solution

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Automated Purification of Antibodies Using ÄKTA Chromatography Systems

Chapter 5, Extracted from Affinity Chromatography Vol. 1: Antibodies, GE Healthcare, 2016  
Keywords: Affinity chromatography, Chromatography, Detection methods, Fractionation, Purification

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer ...
Keywords: Absorption, Affinity chromatography, Buffers, Centrifugation, Chromatography, Detergents, Dialysis, Electrophoresis, Fractionation, Gel electrophoresis, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, PAGE, Precipitation, Sample preparations, Separation, Size-exclusion chromatography, Titrations

General Instructions for Affinity Purification Using HiTrap® Columns

Appendix 4, Extracted from Affinity Chromatography Vol. 1: Antibodies, GE Healthcare, 2016  
Keywords: Affinity chromatography, Buffers, Chromatography, PAGE

Performing a Purification of IgG Antibodies with HiTrap® Protein G HP Columns

HiTrap® Protein G HP (Figure 3.10) is a convenient, ready-to-use column prepacked with Protein G Sepharose® High Performance. The columns are available in 1 ml and 5 ml sizes. In common with all HiTr...
Keywords: Affinity chromatography, Buffers, Cell culture, Chromatography, Immunodiffusion, Ligands, PAGE, Sample preparations

Performing a Purification of IgM Antibodies with HiTrap™ IgM Purification HP

HiTrap™ IgM Purification HP 1 ml columns are prepacked with a thiophilic adsorption medium (2-mercaptopyridine coupled to Sepharose® High Performance). The binding capacity of HiTrap™ IgM Purification ...
Keywords: Adsorption, Affinity chromatography, Cell culture, Chromatography, Enzyme-linked immunosorbent assay, Ligands, PAGE, Precipitation, Purification, Sample preparations

Selection Guide for Polymeric Resins in Chromatography & Purification

Sigma-Aldrich carries a vast array of resins, from leading manufacturers; such as, Dow Chemical, Rohm & Haas, Mitsubishi, and Lanxess. Our product line includes adsorbent, ion-exchange (anion / catio...
Keywords: Adsorption, Antibiotics, Combinatorial synthesis, Detergents, Fermentation, Filtration, Hormones, Ion Exchange, Ion exchange resins, Pharmaceutical, Purification, Separation

Two-step Purification of Mouse Monoclonal IgG1 using HiTrap Protein G HP for the capture step

This case shows a purification method for mouse monoclonal IgG from cell culture supernatant using HiTrap Protein G HP for the initial capture. Polishing was performed in the second, SEC step on HiLoa...
Keywords: Affinity chromatography, Cell culture, Chromatography, Immunoprecipitation, PAGE, Separation, Size-exclusion chromatography

Protocols

Affinity Chromatography Troubleshooting

This section focuses on practical problems that may occur when running a chromatography column. The diagrams below give an indication of how a chromatogram may deviate from the ideal during affnity p...
Keywords: Adsorption, Affinity chromatography, Buffers, Chromatography, Detergents, Digestions, Fractionation, Ligands, PAGE, Precipitation, Purification, Sample preparations, Separation, Solvents

Performing a separation with HiTrap Protein G HP

Centrifuge samples (10 000 g for 10 minutes) to remove cells and debris. Filter through a 0.45 µm filter. If required, adjust sample conditions to the pH and ionic strength of the binding buffer eith...
Keywords: Affinity chromatography, Buffers, Cell culture, Chromatography, Ligands, PAGE

Sample Preparation for Affinity Chromatography

Appendix 1 extracted from Affinity Chromatography Principles and Methods, GE Healthcare, 2007
Keywords: Adsorption, Affinity chromatography, Buffers, Centrifugation, Chromatography, Detergents, Filtration, Nucleic acid denaturation, PAGE, Precipitation, Sample preparations

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