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GE17-0618-01

Protein G Sepharose® 4 Fast Flow

GE Healthcare, 17-0618-01, pack of 5 mL

  •  NACRES NA.56

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Properties

Related Categories Affinity Chromatography, Antibodies, Antibody Purification and Characterization, Heparin, Molecular Biology,
shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging   pack of 5 mL
mfr. no.   GE Healthcare, 17-0618-01
matrix   4% cross-linked agarose
average diameter   90 μm (d50v)
cleaning in place   2 - 10
working range   3 - 9
suitability   suitable for bioprocess medium
storage temp.   2-8°C

Description

General description

Protein G Sepharose® 4 Fast Flow is recombinant protein G coupled to Sepharose® 4 Fast Flow.

Protein G Sepharose® 4 Fast Flow has recombinant protein G immobilized by the cyanogen bromide (CNBr) method to Sepharose® 4 Fast Flow. Protein G exhibit binding specificities that complement Protein A media and binds to the Fc region of IgG from a variety of mammalian species. Protein G Sepharose® 4 Fast Flow may be used to isolate and purify classes, subclasses and fragments of immunoglobulins from any biological fluid or cell culture medium.

As member of the BioProcess media range, Protein G Sepharose® 4 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.

Features and Benefits

• Binding specificities that complement Protein A media.
• Binds a broad range of IgG species and subclasses.
• Multi-point attachment minimizes ligand leakage.
• Used in a range of research applications.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.gelifesciences.com.

Legal Information

Sepharose is a registered trademark of Cytiva

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Column Packing and Preparation for Affinity Chromatography of Antibodies

Appendix 5, Extracted from Affinity Chromatography Vol. 1: Antibodies, GE Healthcare, 2016  
Keywords: Affinity chromatography, Antimicrobials, Chromatography, Detergents, Gene expression, Separation, Solvents

Converting from Flow Velocity to Volumetric Flow Rates

Appendix 7, Extracted from Affinity Chromatography Vol. 1: Antibodies, GE Healthcare, 2016  
Keywords: Affinity chromatography, Chromatography

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer ...
Keywords: Absorption, Affinity chromatography, Buffers, Centrifugation, Chromatography, Detergents, Dialysis, Electrophoresis, Fractionation, Gel electrophoresis, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, PAGE, Precipitation, Sample preparations, Separation, Size-exclusion chromatography, Titrations

Immunoprecipitation Techniques

Appendix 3, Extracted from Affinity Chromatography Vol. 1: Antibodies, GE Healthcare, 2016  
Keywords: Affinity chromatography, Buffers, Cell disruption, Chromatography, Detergents, Electrophoresis, Enzyme activity, Gel electrophoresis, Homogenization, Immobilization, Immunoprecipitation, Mass spectrometry, PAGE, Sample preparations, Separation, Sonication, Western blot

Performing a Purification of IgG Antibodies with Protein G Sepharose® 4 Fast Flow

Protein G Sepharose® 4 Fast Flow consists of 90 µm beads of highly cross-linked agarose, which provide a robust and stable chromatography matrix that allows high flow rates. The medium is a good choi...
Keywords: Affinity chromatography, Buffers, Chromatography, Purification, Sample preparations, Separation

Protein G and protein A bind to different IgG

The high affinity of protein G and protein A for the Fc region of polyclonal and monoclonal IgG-type antibodies forms the basis for purification of IgG, IgG fragments containing the Fc region, and Ig...
Keywords: Adsorption, Affinity chromatography, Cell culture, Chromatography, Enzyme-linked immunosorbent assay, Ion Exchange, Purification

Protocols

Affinity Chromatography Troubleshooting

This section focuses on practical problems that may occur when running a chromatography column. The diagrams below give an indication of how a chromatogram may deviate from the ideal during affnity p...
Keywords: Adsorption, Affinity chromatography, Buffers, Chromatography, Detergents, Digestions, Fractionation, Ligands, PAGE, Precipitation, Purification, Sample preparations, Separation, Solvents

Performing a separation with HiTrap Protein G HP

Centrifuge samples (10 000 g for 10 minutes) to remove cells and debris. Filter through a 0.45 µm filter. If required, adjust sample conditions to the pH and ionic strength of the binding buffer eith...
Keywords: Affinity chromatography, Buffers, Cell culture, Chromatography, Ligands, PAGE

Pull-down assays

Extracted from Purifying Challenging Proteins - Principles and Methods, GE Healthcare, 2007
Keywords: Buffers, Cell disruption, Centrifugation, Chromatography, Cloning, Digestions, Gene expression, Genetic, Immobilization, Immunoprecipitation, Ligands, Mass spectrometry, PAGE, Precipitation

Sample Preparation for Affinity Chromatography

Appendix 1 extracted from Affinity Chromatography Principles and Methods, GE Healthcare, 2007
Keywords: Adsorption, Affinity chromatography, Buffers, Centrifugation, Chromatography, Detergents, Filtration, Nucleic acid denaturation, PAGE, Precipitation, Sample preparations

Related Content

Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary, Secondary and Recombinant Monoclonal Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay...
Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot

Peer-Reviewed Papers
15

References

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