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GE17-0885-02

Gammabind G Sepharose®

GE Healthcare, 17-0885-02, pack of 25 mL

  •  NACRES NA.56

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Properties

Related Categories Affinity Chromatography, Antibodies, Antibody Purification and Characterization, Avidin Biotin Matrices, Molecular Biology,
shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging   pack of 25 mL
mfr. no.   GE Healthcare, 17-0885-02
matrix   4% agarose
particle size   45-165 μm
average diameter   90 μm
cleaning in place   2 - 9 (pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)
working range   3 - 9
capacity   >17 mg binding capacity(human IgG/ml)
storage temp.   2-8°C

Description

General description

Recombinant GammaBind is an affinity medium for laboratory scale antibody purification. Binds to all IgG subclasses from human, mouse, and rat; binds total IgG from guinea pig, rabbit, goat, cow, sheep, and horse.

GammaBind G Sepharose® is GammaBind G, Type 2, covalently immobilized to Sepharose® 4B by the cyanogen bromide method. GammaBind G, Type 2, a recombinant form of streptococcal Protein G, binds to the Fc region of IgG from a variety of mammalian species. GammaBind G Sepharose® may be used to analyze and purify classes, subclasses and fragments of immunoglobulins from any biological fluid or cell culture medium.

GammaBind G Sepharose® may be used to analyze and purify classes, subclasses and fragments of immunoglobulins from any biological fluid or cell culture medium. Since only the Fc region is involved in binding, the Fab region is still available for binding antigen. Hence, GammaBind G Sepharose® is extremely useful for isolation of immune complexes. GammaBind G will not cross-react with other serum proteins such as IgM, IgE, IgA or transferrin.

Application

The potential applications of GammaBind G Sepharose® include practically all of the current and projected applications of Protein A Sepharose. However, GammaBind G and Protein A have different IgG binding specificities, depending on the origin of the IgG. Compared to Protein A, GammaBind G binds more strongly to mouse and rat monoclonal IgG and to IgG from goat, sheep, horse, cow, human, rabbit and other mammalian species.

Features and Benefits

• Recombinant GammaBind G type 2 coupled to Sepharose® 4B engineered for antibody purification at laboratory scale.
• Binds to all IgG subclasses from human, mouse, and rat.
• Binds total IgG from guinea pig, rabbit, goat, cow sheep, and horse.
• Will not cross-react with other serum proteins such as IgM, IgE, IgA or transferrin.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.gelifesciences.com.

Legal Information

Sepharose is a registered trademark of Cytiva

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

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