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GE17-1128-01

MAbtrap® Kit

GE Healthcare, 17-1128-01, pkg of 1 kit

  •  NACRES NA.56

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Properties

shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging   pkg of 1 kit
mfr. no.   GE Healthcare, 17-1128-01
parameter   <4 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
  42 psi
bed size   7 mm × 25 mm
bed volume   1 mL
column I.D.   7 mm
matrix   highly cross-linked 6% agarose
avg. part. size   34 μm
cleaning   2 - 9 (pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)
working range   3 - 9 (pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)
capacity   >1 mg binding capacity (lgG/column)(The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
storage temp.   2-8°C

Description

General description

MAbTrap® is an affinity chromatography kit, with everything required for small-scale IgG purification from ascites fluid, serum, and cell culture supernatants.

Application

MAbTrap® Kit is an affinity chromatography kit for fast and effective purification of monoclonal and polyclonal IgG from ascites fluid, serum, and cell culture supernatants. MAbTrap® Kit contains everything necessary to perform a complete one step purification without using complicated equipment. It also eliminates time-consuming buffer preparation and column packing. The kit includes one HiTrap® column, prepacked with Protein G Sepharose® High Performance.

Features and Benefits

• Convenient kit for purification of IgG from monoclonal and polyclonal material using a syringe in less than 15 min.
• Kit contains one HiTrap® Protein G HP column prepacked with Protein G Sepharose® High Performance and enough buffer concentrate for 20 preparations with a syringe.
• MAbTrap® Kit contains everything necessary to perform a complete one-step purification without using complex equipment.

Analysis Note

www.gelifesciences.com.

Legal Information

HiTrap is a registered trademark of Cytiva

MAbTrap is a registered trademark of Cytiva

Sepharose is a registered trademark of Cytiva

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Performing a Purification of IgG Antibodies with HiTrap® Protein G HP Columns

HiTrap® Protein G HP (Figure 3.10) is a convenient, ready-to-use column prepacked with Protein G Sepharose® High Performance. The columns are available in 1 ml and 5 ml sizes. In common with all HiTr...
Keywords: Affinity chromatography, Buffers, Cell culture, Chromatography, Immunodiffusion, Ligands, PAGE, Sample preparations

Performing a Purification of IgG Antibodies with MabTrap® Kit

MAbTrap® Kit contains one HiTrap® Protein G HP 1 ml column, binding, elution, and neutralization buffers, a syringe with fittings, and an optimized purification protocol (Figure 3.12). The kit contains...
Keywords: Affinity chromatography, Buffers, Cell culture, Chromatography, PAGE, Sample preparations

Protocols

Affinity Chromatography Troubleshooting

This section focuses on practical problems that may occur when running a chromatography column. The diagrams below give an indication of how a chromatogram may deviate from the ideal during affnity p...
Keywords: Adsorption, Affinity chromatography, Buffers, Chromatography, Detergents, Digestions, Fractionation, Ligands, PAGE, Precipitation, Purification, Sample preparations, Separation, Solvents

Performing a separation with HiTrap Protein G HP

Centrifuge samples (10 000 g for 10 minutes) to remove cells and debris. Filter through a 0.45 µm filter. If required, adjust sample conditions to the pH and ionic strength of the binding buffer eith...
Keywords: Affinity chromatography, Buffers, Cell culture, Chromatography, Ligands, PAGE

Sample Preparation for Affinity Chromatography

Appendix 1 extracted from Affinity Chromatography Principles and Methods, GE Healthcare, 2007
Keywords: Adsorption, Affinity chromatography, Buffers, Centrifugation, Chromatography, Detergents, Filtration, Nucleic acid denaturation, PAGE, Precipitation, Sample preparations

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