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GE17-1279-01

rProtein A Sepharose® Fast Flow

GE Healthcare, 17-1279-01, pack of 5 mL

  •  NACRES NA.56

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Properties

Related Categories Antibodies, Antibody Purification and Characterization, Protein A, G and L Resins, Supplementary Products
shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging   pack of 5 mL
mfr. no.   GE Healthcare, 17-1279-01
matrix   4% cross-linked agarose
particle size   60-165 μm
average diameter   90 μm (d50v)
cleaning in place   2 - 11
working range   3 - 10
capacity   ~50 mg binding capacity (human lgG/ml medium)(Capacity of HiTrap rProtein A for some monoclonal antibodies. Running conditions: Binding buffer: 20 mM sodium phosphate (incl. 3 M NaCl for IgG), pH 7.0. Elution buffer: 0.1 M sodium citrate, pH 3.0.)
suitability   suitable for bioprocess medium
storage temp.   2-8°C

Description

General description

rProtein A Sepharose® 4 Fast Flow is a well established antibody affinity medium, designed for the purification of monoclonal and polyclonal antibodies at both laboratory and process scale.

rProtein A Sepharose® 4 Fast Flow is based on the well established Sepharose® Fast Flow platform and is used in many approved monoclonal antibody (MAb) processes. It is composed of recombinant protein A coupled to the cross-linked 4% agarose base matrix. The recombinant protein A is produced in E. coli and has been specially engineered to favour an oriented coupling giving a matrix with enhanced binding capacity. The epoxy based coupling ensures low ligand leakage. The specificity of the recombinant protein A for the Fc region of IgG is similar to native protein A, giving a high purification factor in a single step. The high capacity, low ligand leakage and a well established base matrix makes rProtein A Sepharose® 4 Fast Flow suitable for purification of monoclonal antibodies at both laboratory and process scale.

rProtein A Sepharose® 4 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As member of the BioProcess media range, rProtein A Sepharose® 4 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.

Features and Benefits

• High dynamic binding capacity due to oriented, single-point attachment of protein A
• Well-established Protein A medium used in many approved MAb processes
• No mammalian components involved during the manufacturing process

Analysis Note

To view the Certificate of Analysis for this product, please visit www.gelifesciences.com.

Legal Information

Sepharose is a registered trademark of Cytiva

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Column Packing and Preparation for Affinity Chromatography of Antibodies

Appendix 5, Extracted from Affinity Chromatography Vol. 1: Antibodies, GE Healthcare, 2016  
Keywords: Affinity chromatography, Antimicrobials, Chromatography, Detergents, Gene expression, Separation, Solvents

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer ...
Keywords: Absorption, Affinity chromatography, Buffers, Centrifugation, Chromatography, Detergents, Dialysis, Electrophoresis, Fractionation, Gel electrophoresis, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, PAGE, Precipitation, Sample preparations, Separation, Size-exclusion chromatography, Titrations

Immunoprecipitation Techniques

Appendix 3, Extracted from Affinity Chromatography Vol. 1: Antibodies, GE Healthcare, 2016  
Keywords: Affinity chromatography, Buffers, Cell disruption, Chromatography, Detergents, Electrophoresis, Enzyme activity, Gel electrophoresis, Homogenization, Immobilization, Immunoprecipitation, Mass spectrometry, PAGE, Sample preparations, Separation, Sonication, Western blot

Performing a Purification of IgG Antibodies with Protein G Sepharose® 4 Fast Flow

Protein G Sepharose® 4 Fast Flow consists of 90 µm beads of highly cross-linked agarose, which provide a robust and stable chromatography matrix that allows high flow rates. The medium is a good choi...
Keywords: Affinity chromatography, Buffers, Chromatography, Purification, Sample preparations, Separation

Performing a Purification of IgG Antibodies with rProtein A Sepharose Fast Flow

rProtein A Sepharose® Fast Flow (Figure 3.19) is an affinity medium with high binding capacity for monoclonal and polyclonal antibodies. The binding capacity of rProtein A Sepharose® Fast Flow is cons...
Keywords: Affinity chromatography, Buffers, Chromatography, Fermentation, Ligands, Sample preparations

Purification using protein A chromatography media

Protein A is derived from a strain of Staphylococcus aureus and contains five regions that bind to the Fc region of IgG. As an affinity ligand, protein A is coupled to Sepharose® so that these region...
Keywords: Affinity chromatography, Buffers, Chromatography, Fermentation, Immunoprecipitation, Ligands, Purification, Size-exclusion chromatography

Protocols

Affinity Chromatography Troubleshooting

This section focuses on practical problems that may occur when running a chromatography column. The diagrams below give an indication of how a chromatogram may deviate from the ideal during affnity p...
Keywords: Adsorption, Affinity chromatography, Buffers, Chromatography, Detergents, Digestions, Fractionation, Ligands, PAGE, Precipitation, Purification, Sample preparations, Separation, Solvents

Buffer Exchange and Desalting for Affinity Chromatography

Dialysis is frequently mentioned in the literature as a technique to remove salt or other small molecules and to exchange the buffer composition of a sample. However, dialysis is generally a very slo...
Keywords: Absorption, Affinity chromatography, Buffers, Chromatography, Dialysis, Separation

Column Cleaning for Ion Exchange Chromatography and Chromatofocusing

Appendix 10, extracted from Ion Exchange Chromatography & Chromatofocusing, GE Healthcare, 2007
Keywords: Buffers, Chromatography, Detergents, Ion Exchange, Separation, Solvents

Column Packing and Preparation for Affinity Chromatography

Appendix 3,  Extracted from Affinity Chromatography Principles and Methods, GE Healthcare, 2007
Keywords: Affinity chromatography, Antimicrobials, Buffers, Chromatography, Separation

Converting From Linear Fow (cm/hour) to Volumetric Flow Rates (ml/min) and Vice Versa

Appendix 4 extracted from Affinity Chromatography Principles and Methods, GE Healthcare, 2007
Keywords: Affinity chromatography, Chromatography

Performing a Separation with GE Healthcare Products Based on Protein A

Protein A is derived from a strain of Staphylococcus aureus and contains five regions that bind to the Fc region of IgG. As an affinity ligand, protein A is coupled to Sepharose so that these regions a...
Keywords: Affinity chromatography, Buffers, Cell culture, Chromatography, Ligands, PAGE, Purification

Sample Preparation for Affinity Chromatography

Appendix 1 extracted from Affinity Chromatography Principles and Methods, GE Healthcare, 2007
Keywords: Adsorption, Affinity chromatography, Buffers, Centrifugation, Chromatography, Detergents, Filtration, Nucleic acid denaturation, PAGE, Precipitation, Sample preparations

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