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  • GE17-1279-02 - rProtein A Sepharose® Fast Flow


rProtein A Sepharose® Fast Flow

GE Healthcare, 17-1279-02, pack of 25 mL

  •  NACRES NA.56



Related Categories Antibodies, Antibody Purification and Characterization, Protein A, G and L Resins, Supplementary Products
shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging   pack of 25 mL
mfr. no.   GE Healthcare, 17-1279-02
matrix   4% cross-linked agarose
particle size   60-165 μm
average diameter   90 μm (d50v)
cleaning in place   2 - 11
working range   3 - 10
capacity   ~50 mg binding capacity (human lgG/ml medium)(Capacity of HiTrap rProtein A for some monoclonal antibodies. Running conditions: Binding buffer: 20 mM sodium phosphate (incl. 3 M NaCl for IgG), pH 7.0. Elution buffer: 0.1 M sodium citrate, pH 3.0.)
suitability   suitable for bioprocess medium
storage temp.   2-8°C


General description

rProtein A Sepharose® 4 Fast Flow is a well established antibody affinity medium, designed for the purification of monoclonal and polyclonal antibodies at laboratory scale.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.gelifesciences.com.

Legal Information

Sepharose is a registered trademark of Cytiva

Safety & Documentation

Safety Information

NONH for all modes of transport


Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Column Cleaning for Ion Exchange Chromatography and Chromatofocusing

Appendix 10, extracted from Ion Exchange Chromatography & Chromatofocusing, GE Healthcare, 2007
Keywords: Buffers, Chromatography, Detergents, Ion Exchange, Separation, Solvents

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