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GE17-5138-01

rmp Protein A Sepharose® Fast Flow

GE Healthcare, 17-5138-01, pack of 5 mL

  •  NACRES NA.56

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Properties

Related Categories Antibodies, Antibody Purification and Characterization, Protein A, G and L Resins, Supplementary Products
shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging   pack of 5 mL
mfr. no.   GE Healthcare, 17-5138-01
matrix   4% cross-linked agarose
particle size   60-165 μm
average diameter   90 μm (d50v)
cleaning in place   2 - 11
working range   3 - 10
capacity   >35 mg binding capacity(human IgG/ml)
suitability   suitable for bioprocess medium
storage temp.   2-8°C

Description

General description

rmp protein A Sepharose® Fast FLow is a low leakage, non-mammalian based affinity medium designed for high purity separation of monoclonal and polyclonal antibodies at the laboratory and process scales.

Application

rmp Sepharose® 4 Sepharose® Fast FLow is composed of recombinant Protein A coupled to the well established Sepharose® 4 Fast FLow base matrix by reductive amination resulting in multipoint attachment.

Reductive amination creates chemically stable amide bonds and resulting Low ligand leakage. rmp Protein A Sepharose® 4 Fast FLow is manufactured without using animal-derived components. As member of the BioProcess media range, rmp Sepharose® 4 Fast FLow meets industrial demands with security of supply and comprehensive technical and regulatory support.

Features and Benefits

• Multi-point attachment of rProtein A by chemically-stable amide bonds
• Coupling chemistry that minimizes leakage of Protein A ligand.

Analysis Note

www.gelifesciences.com.

Legal Information

Sepharose is a registered trademark of Cytiva

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Column Cleaning for Ion Exchange Chromatography and Chromatofocusing

Appendix 10, extracted from Ion Exchange Chromatography & Chromatofocusing, GE Healthcare, 2007
Keywords: Buffers, Chromatography, Detergents, Ion Exchange, Separation, Solvents

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