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rmp Protein A Sepharose® Fast Flow

Cytiva, 17-5138-03, pack of 200 mL

  •  NACRES NA.56



Related Categories Antibodies, Antibody Purification and Characterization, Protein A, G and L Resins, Supplementary Products
shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging   pack of 200 mL
mfr. no.   Cytiva, 17-5138-03
matrix   4% cross-linked agarose
particle size   60-165 μm
average diameter   90 μm (d50v)
cleaning in place   2 - 11
working range   3 - 10
capacity   >35 mg binding capacity(human IgG/ml)
suitability   suitable for bioprocess medium
storage temp.   2-8°C


General description

rmp Protein A Sepharose® Fast Flow is a low leakage, non-mammalian based affinity medium designed for high purity separation of monoclonal and polyclonal antibodies at the laboratory and process scales.

rmp Sepharose® 4 Sepharose® Fast Flow is composed of recombinant Protein A coupled to the well established Sepharose® 4 Fast Flow base matrix by reductive amination resulting in multipoint attachment.

Reductive amination creates chemically stable amide bonds and resulting low ligand leakage. rmp Protein A Sepharose® 4 Fast Flow is manufactured without using animal-derived components.

As member of the BioProcess media range, rmp Sepharose® 4 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.

Features and Benefits

• Multi-point attachment of rProtein A by chemically-stable amide bonds
• Coupling chemistry that minimizes leakage of protein A ligand.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.gelifesciences.com.

Legal Information

Sepharose is a registered trademark of Cytiva

Safety & Documentation

Safety Information

NONH for all modes of transport


Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
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Column Cleaning for Ion Exchange Chromatography and Chromatofocusing

Appendix 10, extracted from Ion Exchange Chromatography & Chromatofocusing, GE Healthcare, 2007
Keywords: Buffers, Chromatography, Detergents, Ion Exchange, Separation, Solvents

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