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GE17-5199-01

MabSelect

GE Healthcare, 17-5199-01, pack of 25 mL

  •  NACRES NA.56

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Properties

Related Categories Antibodies, Antibody Purification and Characterization, MabSelect mAb Purification, Protein A, G and L Resins, Supplementary Products More...
shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging   pack of 25 mL
mfr. no.   GE Healthcare, 17-5199-01
matrix   highly cross-linked agarose
avg. part. size   85 μm (d₅₀v is the median particle size of the cumulative volume distribution)
cleaning in place   2 - 12 (pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)
working range   3 - 10
capacity   ≥30 mg binding capacity (humanlgG/ml at 2.4 min residence time)(Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 500 cm/h in a column with a bed height of 20 cm.)
suitability   suitable for bioprocess medium
storage temp.   2-8°C

Description

General description

MabSelect is a protein A affinity medium designed for capturing MAbs from large sample volumes.

Application

MabSelect is a BioProcess affinity medium for capturing monoclonal antibodies (MAbs) from large sample volumes. MabSelect is developed for cost-effective high throughput processing of large amounts of feed. This is the reason why MabSelect has been designed-in to several new and second-generation processes for the production of therapeutic MAbs.

The medium is based on a highly cross-linked Agarose matrix with a recombinant Protein A ligand. This ligand is engineered to favor an oriented coupling resulting in an affinity medium with enhanced binding capacity for IgG. The hydrophilic nature of the base matrix ensures Low levels of non-specific binding leading to Low levels of host cell-derived impurities in the elution pool. MabSelect is optimized for high capacity, throughput and to deliver a product pool that is high in purity and yield.

The recombinant Protein A ligand is expressed in E. coli and is free of components of mammalian origin. The specificity of binding to the Fc region of IgG is similar to that of native Protein A giving a high purification factor in a single step. In addition, the high capacity and the novel base matrix make MabSelect an excellent choice for purification of MAbs at process scale.

MabSelect is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As member of the BioProcess media range, MabSelect meets industrial demands with security of supply and comprehensive technical and regulatory support.

Features and Benefits

• High-fLow Agarose matrix allows for high flow velocities at production scale enabling the processing of more than 10 000 L of feed in one working day.
• Oriented coupling of the ligand and optimized matrix gives high dynamic binding capacity which reduces media volume requirements
• Low non-specific binding resulting in Low levels of impurities in the product eluate pool
• BioProcess medium supported for industrial applications and well-established in approved processes.

Analysis Note

www.gelifesciences.com.

Legal Information

MabSelect is a trademark of Cytiva

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Column Packing and Preparation for Affinity Chromatography of Antibodies

Appendix 5, Extracted from Affinity Chromatography Vol. 1: Antibodies, GE Healthcare, 2016  
Keywords: Affinity chromatography, Antimicrobials, Chromatography, Detergents, Gene expression, Separation, Solvents

Packing Tricorn 10/100 columns with MabSelect™ or MabSelect™ SuRe

Ensure that back pressure does not exceed the pressure limits (< 5 MPa) of the column during packing.
Keywords: Affinity chromatography, Chromatography

Protocols

Affinity Chromatography Troubleshooting

This section focuses on practical problems that may occur when running a chromatography column. The diagrams below give an indication of how a chromatogram may deviate from the ideal during affnity p...
Keywords: Adsorption, Affinity chromatography, Buffers, Chromatography, Detergents, Digestions, Fractionation, Ligands, PAGE, Precipitation, Purification, Sample preparations, Separation, Solvents

Bind/Elute vs Flowthrough Mode in Multimodal Chromatography

In multimodal chromatography, the choice between bind/elute and flowthrough mode is more complex than when using a single method, such as IEX, because multiple types of interactions are occurring in ...
Keywords: Chromatography, Indicators

Buffer Exchange and Desalting for Affinity Chromatography

Dialysis is frequently mentioned in the literature as a technique to remove salt or other small molecules and to exchange the buffer composition of a sample. However, dialysis is generally a very slo...
Keywords: Absorption, Affinity chromatography, Buffers, Chromatography, Dialysis, Separation

Capto Adhere

Capto adhere is a multimodal strong anion exchanger for BioProcess applications. It was originally designed for post-protein A purification of MAbs at process scale in flowthrough mode. However, Capt...
Keywords: Cell culture, Chromatography, Deamidations, Ion Exchange, Ligands, Purification, Reductions, Separation

Column Packing and Preparation for Affinity Chromatography

Appendix 3,  Extracted from Affinity Chromatography Principles and Methods, GE Healthcare, 2007
Keywords: Affinity chromatography, Antimicrobials, Buffers, Chromatography, Separation

Converting From Linear Fow (cm/hour) to Volumetric Flow Rates (ml/min) and Vice Versa

Appendix 4 extracted from Affinity Chromatography Principles and Methods, GE Healthcare, 2007
Keywords: Affinity chromatography, Chromatography

HTS and Optimization of a Multimodal Polishing Step in a MAb Purification Process Using Capto Adhere

This application describes the use of Capto adhere and MabSelect SuRe chromatography media to significantly reduce the level of IgG antibody aggregates in a sample using an efficient two-step method....
Keywords: Adsorption, Buffers, Centrifugation, Chromatography, Enzyme-linked immunosorbent assay, Filtration, Ion Exchange, Ligands, Mass spectrometry, Purification, Reductions

Maintenance of Multimodal Chromatography Media and Storage Conditions

For best performance of multimodal chromatography media over a long working lifetime, follow the procedures described below.
Keywords: Antimicrobials, Chromatography, Ligands, Purification, Reductions, Solvents

Optimization of Loading Conditions on Capto Adhere Using DoE

This study describes the optimization of loading conditions for a MAb polishing step to obtain the window of operation for Capto adhere. In order to find the optimal conditions, a full factorial DoE ...
Keywords: Chromatography, Mass spectrometry, Purification

Performing a Separation with GE Healthcare Products Based on Protein A

Protein A is derived from a strain of Staphylococcus aureus and contains five regions that bind to the Fc region of IgG. As an affinity ligand, protein A is coupled to Sepharose so that these regions a...
Keywords: Affinity chromatography, Buffers, Cell culture, Chromatography, Ligands, PAGE, Purification

Sample Preparation for Affinity Chromatography

Appendix 1 extracted from Affinity Chromatography Principles and Methods, GE Healthcare, 2007
Keywords: Adsorption, Affinity chromatography, Buffers, Centrifugation, Chromatography, Detergents, Filtration, Nucleic acid denaturation, PAGE, Precipitation, Sample preparations

Use of Multimodal Chromatography Media for MAb Purification

Typical MAb purification processes consist of capture by protein A followed by one or two polishing steps (Fig A3.1). MabSelect SuRe is based on a protein A derivative with higher alkali stability co...
Keywords: Chromatography, Ion Exchange, Ligands, Purification, Reductions

Viral Clearance Using Capto Adhere

Viral clearance using Capto adhere was tested with two representative model viruses, Minute Virus of Mice (MVM) and Murine Leukemia Virus (MuLV). Monoclonal IgG1 was purified from CHO cell supernatan...
Keywords: Chromatography, Mass spectrometry, Reductions

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