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GE17-5318-01

Ni Sepharose® 6Fast Flow

GE Healthcare, 17-5318-01, pack of 25 mL

  •  NACRES NA.56

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Properties

Related Categories Affinity Chromatography, Heparin, IMAC Matrices, Molecular Biology, Protein Chromatography,
shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging   pack of 25 mL
mfr. no.   GE Healthcare, 17-5318-01
matrix   6% cross-linked agarose
particle size   45-165 μm
average diameter   90 μm
cleaning in place   2 - 14 (Ni2+-stripped medium.)
working range   3 - 12 (Ni2+-stripped medium.)
capacity   ~40 mg binding capacity(histidine-tagged protein)
suitability   suitable for bioprocess medium

Description

General description

Ni Sepharose® 6 Fast Flow is a BioProcess medium that combines the advantages of Ni?? for purification of histidine tagged proteins, with the well industrially established properties of the Sepharose® Fast Flow platform.

Ni Sepharose® 6 Fast Flow is a member of the GE Healthcare range of IMAC chromatography media for capture and intermediate purification.

Ni Sepharose® 6 Fast Flow is composed of cross-linked 6% agarose beads modified with a novel chelating ligand immobilized to the base matrix and pre-charged with Ni2? ions for purification of histidine tagged proteins. The coupling chemistry and the novel ligand is designed to give minimal ligand and Ni2? leakage while providing high capacity.

Ni Sepharose® 6 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As member of the BioProcess media range, Ni Sepharose® 6 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.

Features and Benefits

• Designed for scaling up purification of histidine-tagged proteins
• High protein binding capacity and minimal leakage of Ni2? ions
• As BioProcess medium it fulfills industrial demands for security of supply, robust performance and regulatory support.
• Available in convenient prepacked HisPrep and HisTrap column formats.

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.gelifesciences.com.

Legal Information

IMAC Sepharose products, Ni Sepharose products and Fe Sepharose products
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.

HisPrep is a trademark of Cytiva

HisTrap is a trademark of Cytiva

Sepharose is a registered trademark of Cytiva

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Converting from Flow Velocity to Volumetric Flow Rates

Appendix 7, Extracted from Affinity Chromatography Vol. 1: Antibodies, GE Healthcare, 2016  
Keywords: Affinity chromatography, Chromatography

Protocols

Characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and Uncharged IMAC Sepharose Products

Appendix 1, Extracted from Affinity Chromatography Vol. 2: Tagged Proteins, GE Healthcare, 2016  
Keywords: Affinity chromatography, Buffers, Cell culture, Centrifugation, Chromatography, Detergents, Immobilization, Precipitation, Purification, Tagged proteins

Column Packing and Preparation for Affinity Chromatography with Specific Groups of Biomolecules

Appendix 3, extracted from Affinity Chromatography Vol. 3: Specific Groups of Biomolecules, GE Healthcare, 2014
Keywords: Antimicrobials, Chromatography, Gene expression, Separation

Column Packing and Preparation

Appendix 6, Extracted from Affinity Chromatography Vol. 2: Tagged Proteins, GE Healthcare, 2016  
Keywords: Affinity chromatography, Antimicrobials, Chromatography, Gene expression, Separation, Tagged proteins

Condition screening for scaling up using HiScreen™ Ni FF

HiScreen Ni FF is a ready-to-use column for purification of histidine-tagged recombinant proteins by IMAC. The column has a volume of 4.7 ml of Ni Sepharose 6 Fast Flow and is well suited for screenin...
Keywords: Affinity chromatography, Buffers, Chromatography, PAGE, Tagged proteins

Desalting/Buffer Exchange and Concentration for Affinity Chromatography of Tagged Proteins

Desalting at laboratory scale is a well-proven, simple, and very fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired bu...
Keywords: Absorption, Addition reactions, Affinity chromatography, Buffers, Centrifugation, Chromatography, Detergents, Dialysis, Fractionation, Ion Exchange, PAGE, Precipitation, Sample preparations, Separation, Size-exclusion chromatography, Tagged proteins

Gravity-flow purification using His GraviTrap and His GraviTrap Kit

His GraviTrap™ columns are designed for fast and simple purification of histidine-tagged proteins using gravity flow. Both clarified and unclarified sample can be applied to the column. The column is pre...
Keywords: Affinity chromatography, Buffers, Cell disruption, Chromatography, PAGE, Precipitation, Sequencing, Sonication, Tagged proteins, Western blot

Manual purification using HisTrap FF crude Kit with a syringe

HisTrap™ FF crude Kit is designed for rapid and convenient manual purification of histidine-tagged proteins using premade buffers and a syringe. Histidine-tagged proteins can be purified directly from ...
Keywords: Affinity chromatography, Buffers, Cell disruption, Chromatography, Homogenization, PAGE, Precipitation, Sample preparations, Sonication, Tagged proteins, Western blot

Optimizing Purification of Histidine-Tagged Proteins

Chapter 4, Extracted from Affinity Chromatography Vol. 2: Tagged Proteins, GE Healthcare, 2016
Keywords: Affinity chromatography, Buffers, Cell disruption, Chromatography, Immobilization, Ion Exchange, PAGE, Purification, Separation, Sequencing, Size-exclusion chromatography, Tagged proteins

Performing High-Throughput Screening Using His MultiTrap HP and His MultiTrap FF 96-Well filter Plates

His MultiTrap™ HP and His MultiTrap™ FF are prepacked, disposable 96-well filter plates for reproducible, high-throughput screening of histidine-tagged recombinant protein expression. Typical applicat...
Keywords: Affinity chromatography, Biochemistry, Buffers, Cell disruption, Centrifugation, Chromatography, Detergents, Dot blot, Gene expression, PAGE, Precipitation, Size-exclusion chromatography, Tagged proteins

Performing a Cell Lysis of Recombinant Histidine-Tagged Proteins

For optimal conditions for growth, induction, and cell lysis of recombinant histidine-tagged proteins, please refer to established procedures. The following is a general procedure for cell lysis and ...
Keywords: Affinity chromatography, Buffers, Cell disruption, Centrifugation, Chromatography, Filtration, Homogenization, Precipitation, Sample preparations, Sonication, Tagged proteins

Principles and Standard Conditions for Different Purification Techniques

Appendix 11, Extracted from Affinity Chromatography Vol. 2: Tagged Proteins, GE Healthcare, 2016
Keywords: Affinity chromatography, Chromatography, Detergents, Ion Exchange, Ligands, Precipitation, Reversed-phase chromatography, Separation, Size-exclusion chromatography, Solvents, Tagged proteins

Purification or Removal of Proteins and Peptides with Exposed Amino Acids: His, Cys, Trp, and/or with Affinity for Metal Ions

Extracted from Affinity Chromatography Vol. 3: Specific Groups of Biomolecules, GE Healthcare, 2014
Keywords: Adsorption, Buffers, Chromatography, Detergents, Immobilization, Ion Exchange, Ligands, Phase transitions, Precipitation, Reductions, Separation, Tagged proteins

Purification Using HisTrap HP and HisTrap FF

HisTrap HP and HisTrap FF are 1 ml and 5 ml HiTrap columns packed with Ni Sepharose High Performance or Ni Sepharose 6 Fast Flow, respectively. Sample application, washing,  and elution can be perfor...
Keywords: Affinity chromatography, Buffers, Chromatography, PAGE, Purification, Size-exclusion chromatography, Tagged proteins

Purification from unclarified cell lysate using HisTrap FF crude

HisTrap™ FF crude is a ready-to-use column, prepacked with Ni Sepharose® 6 Fast Flow, for purification of histidine-tagged recombinant proteins. After thorough cell disruption, it is possible to load ...
Keywords: Affinity chromatography, Buffers, Cell disruption, Centrifugation, Chromatography, Gene expression, Homogenization, PAGE, Precipitation, Size-exclusion chromatography, Sonication, Tagged proteins

Purification of Histidine-Tagged Proteins Secreted into Eukaryotic Cell Culture Supernatants Using HisTrap™ Excel

HisTrap excel 1 ml and 5 ml columns are ready-to-use IMAC columns prepacked with Ni Sepharose excel. The design of the columns in combination with the specific properties of the medium enables fast an...
Keywords: Affinity chromatography, Buffers, Cell culture, Chromatography, Degradations, Gene expression, PAGE, Tagged proteins

Purification of Histidine-Tagged Recombinant Proteins Using Ni Sepharose 6 Fast Flow

Ni Sepharose 6 Fast Flow consists of 90 µm beads of highly cross-linked agarose, to which a chelating ligand has been immobilized and subsequently charged with Ni2+ ions. The ligand density of Ni Sep...
Keywords: Affinity chromatography, Buffers, Cell disruption, Chromatography, Filtration, Homogenization, Immobilization, Ligands, Sample preparations, Sonication, Tagged proteins

Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules

Appendix 1, extracted from Affinity Chromatography Vol. 3: Specific Groups of Biomolecules, GE Healthcare, 2014
Keywords: Absorption, Adsorption, Buffers, Cell culture, Centrifugation, Chromatography, Detergents, Dialysis, Electrophoresis, Filtration, Indicators, Nucleic acid denaturation, Precipitation, Sample preparations, Separation, Tagged proteins

Starting materials needed for the purification of integral membrane proteins for structural and functional studies

Extracted from Purifying Challenging Proteins - Principles and Methods, GE Healthcare, 2007
Keywords: Cloning, Detergents, Gene expression, Glycosylations, PAGE, Positron Emission Tomography, Separation, Tagged proteins

Tag Removal by Enzymatic Cleavage

In most cases, functional tests can be performed using the intact histidine-tagged protein. If removal of the tag is necessary, then procedures similar to GST tag removal can be followed, that is, sp...
Keywords: Affinity chromatography, Cell disruption, Centrifugation, Chromatography, Digestions, PAGE, Purification, Separation, Size-exclusion chromatography, Sonication, Tagged proteins

Troubleshooting Guide for Affinity Chromatography of Tagged Proteins

The troubleshooting guide below addresses problems common to the majority of purification products discussed in this chapter, as well as problems specific to a particular method. In the latter case, th...
Keywords: Addition reactions, Affinity chromatography, Buffers, Cell culture, Cell disruption, Centrifugation, Chromatography, Detergents, Fermentation, PAGE, Precipitation, Protein biosynthesis, Size-exclusion chromatography, Sonication, Tagged proteins, Western blot

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