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GE17-5474-01

MabSelect SuRe LX

GE Healthcare, 17-5474-01, pack of 25 mL

  •  NACRES NA.56

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Properties

Related Categories Antibodies, Antibody Purification and Characterization, MabSelect mAb Purification, Protein A, G and L Resins, Supplementary Products More...
shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging   pack of 25 mL
mfr. no.   GE Healthcare, 17-5474-01
matrix   highly cross-linked agarose
avg. part. size   85 μm (d₅₀v is the median particle size of the cumulative volume distribution)
cleaning in place   2 - 13.7 (pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)
working range   3 - 12 (pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)
capacity   ~60 mg binding capacity(human IgG/ml medium. Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 100 cm/h in a column with a bed height of 20 cm, i.e. residence time is 6.0 min.)
suitability   suitable for bioprocess medium
storage temp.   2-8°C

Description

General description

MabSelect SuRe LX is a protein A affinity medium with very high dynamic binding capacity at extended residence times and is developed for high titer antibody processes. Alkali tolerance, high capacity and low ligand leakage in combination with the rigid base matric makes MabSelect SuRe LX highly suitable for purification of monoclonal anitbodies for clinical applications.

MabSelect SuRe LX is a member of the MabSelect family of affinity chromatography media for the capture of monoclonal antibodies (MAbs) at process scale. MabSelect SuRe LX shares features and benefits of MabSelect and MabSelect SuRe such as a rigid, high-flow agarose matrix that ensures excellent pressure/flow properties, low non-specific binding that leads to low impurity levels in the eluate pool, and an oriented ligand coupling for optimal binding capacity. It also features the same alkali-tolerant rProtein A ligand as MabSelect SuRe. This ligand provides greater stability than conventional protein A-based media in the alkaline conditions used in cleaning-in-place (CIP) and sanitization protocols. The enhanced alkali stability of MabSelect SuRe improves process economy and product quality; cleaning can be performed with cost-effective reagents such as sodium hydroxide eliminating the need for expensive and hazardous cleaning agents such as Gua-HCl. Process economy is also improved by the increased dynamic binding capacity of MabSelect SuRe LX.

Just as for MabSelect SuRe, the novel ligand construct of MabSelect SuRe LX has been proven to have an increased stability towards proteases. Therefore, the level of leakage of the MabSelect SuRe LX ligand during elution is low.

MabSelect SuRe has been demonstrated to give generic elution conditions for different monoclonal antibodies which can be applied also to MabSelect SURe LX since it utilizes the same ligand. This is an advantage when designing generic purification platform processes. The feature is also correlated to the construction of the MabSelect SuRe ligand.

MabSelect SuRe LX is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As member of the BioProcess media range, MabSelect SuRe meets industrial demands with security of supply and comprehensive technical and regulatory support.

Features and Benefits

• Novel, alkali-tolerant rProtein A ligand withstands rigorous CIP and sanitization procedures with 0.1 to 0.5 M NaOH.
• Reduces the risk of product contamination and carryover
• Improves performance and reduces overall costs
• High dynamic binding capacity (DBC) for high titer cultures reduces process time and amount of medium used
• Generic elution profile for different monoclonal antibodies enables platform approach to purification
• High-flow agarose matrix allows the processing of large feed volumes

Analysis Note

To view the Certificate of Analysis for this product, please visit www.gelifesciences.com.

Legal Information

Free MabSelect SuRe Ligand
"MabSelect SuRe Ligand Restricted License" and "Cys-rProtein A Ligand Restricted License" are protected by the following patents and equivalent patents and patent applications in other countries: US 6,399,750 and EP 1123389. A free, non-transferable limited license to use this product for internal analytical purposes only accompanies the purchase of the product from a GE Healthcare company and its licensed distributors. Any other use will require a separate license from a GE Healthcare company.

MabSelect is a trademark of Cytiva

SuRe is a trademark of Cytiva

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
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Protocols

Bind/Elute vs Flowthrough Mode in Multimodal Chromatography

In multimodal chromatography, the choice between bind/elute and flowthrough mode is more complex than when using a single method, such as IEX, because multiple types of interactions are occurring in ...
Keywords: Chromatography, Indicators

Capto Adhere

Capto adhere is a multimodal strong anion exchanger for BioProcess applications. It was originally designed for post-protein A purification of MAbs at process scale in flowthrough mode. However, Capt...
Keywords: Cell culture, Chromatography, Deamidations, Ion Exchange, Ligands, Purification, Reductions, Separation

HTS and Optimization of a Multimodal Polishing Step in a MAb Purification Process Using Capto Adhere

This application describes the use of Capto adhere and MabSelect SuRe chromatography media to significantly reduce the level of IgG antibody aggregates in a sample using an efficient two-step method....
Keywords: Adsorption, Buffers, Centrifugation, Chromatography, Enzyme-linked immunosorbent assay, Filtration, Ion Exchange, Ligands, Mass spectrometry, Purification, Reductions

Maintenance of Multimodal Chromatography Media and Storage Conditions

For best performance of multimodal chromatography media over a long working lifetime, follow the procedures described below.
Keywords: Antimicrobials, Chromatography, Ligands, Purification, Reductions, Solvents

Optimization of Loading Conditions on Capto Adhere Using DoE

This study describes the optimization of loading conditions for a MAb polishing step to obtain the window of operation for Capto adhere. In order to find the optimal conditions, a full factorial DoE ...
Keywords: Chromatography, Mass spectrometry, Purification

Use of Multimodal Chromatography Media for MAb Purification

Typical MAb purification processes consist of capture by protein A followed by one or two polishing steps (Fig A3.1). MabSelect SuRe is based on a protein A derivative with higher alkali stability co...
Keywords: Chromatography, Ion Exchange, Ligands, Purification, Reductions

Viral Clearance Using Capto Adhere

Viral clearance using Capto adhere was tested with two representative model viruses, Minute Virus of Mice (MVM) and Murine Leukemia Virus (MuLV). Monoclonal IgG1 was purified from CHO cell supernatan...
Keywords: Chromatography, Mass spectrometry, Reductions

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