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GE27-0843-01

PreScission Protease

GE Healthcare, 27-0843-01

  •  NACRES NA.56

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Properties

Related Categories Application Index, Biochemicals and Reagents, Enzymes, Inhibitors, and Substrates, Proteases & Protein Sequencing, Proteases for Recombinant Protein Fusion Tag Cleavage More...
shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
  Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging   pkg of 500 units
mfr. no.   GE Healthcare, 27-0843-01
shipped in   dry ice
storage temp.   −20°C

Description

General description

PreScission Protease is a genetically engineered fusion protein of human rhinovirus 3C protease and glutothione S trasferase (GST). PreScisson protease specifically cleaves between the Gln and Gly residues of the recognition sequence LeuGluValLeuPheGln/GlyPro.

Application

Specific, low temperature cleavage of fusion protein tags; Simple on-column cleavage during affinity purification
PreScission Protease has been used for eluting GST-tagged AT3, during in vitro ubiquitination reactions and AT3-Ub preparation. It has also been used to cleave GST tag from the a-enolase during induction of GST a-enolase expression in the protease-deficient BL21 strain of Escherichia coli.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.gelifesciences.com.

Legal Information

pGEX Vectors
pGEX Vectors are to be used for scientific investigation and research and for no other purpose whatsoever and a license for commercial use of the licensed products and the processes claimed in US patent 5,654,176 and equivalent patents and patent applications in other countries must be negotiated directly with Millipore Corp (formerly Chemicon International Inc) by the purchaser prior to such use.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Protocols

Cleavage and Purification of GST-Tagged Protein Bound to GSTrap

The protocol below is an example optimized for 8 mg of target protein. It is worth estimating how much target protein is applied to the column, as this allows one to minimize the amount of protease a...
Keywords: Affinity chromatography, Buffers, Cell disruption, Centrifugation, Chromatography, Digestions, Filtration, PAGE, Purification, Sonication, Tagged proteins

Cleavage and Purification of GST-Tagged Protein Bound to Glutathione Sepharose in Batch Mode

Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, and Glutathione Sepharose 4B can all be used for cleavage and purification of GST-tagged proteins in batch.
Keywords: Buffers, Cell disruption, Centrifugation, Chromatography, Purification, Tagged proteins

Cleavage and Purification of GST-Tagged Protein Eluted from GSTrap

The protocol below is an example optimized for 8 mg of target protein. It is worth estimating how much target protein is applied to the column, as this allows one to minimize the amount of protease a...
Keywords: Absorption, Buffers, Chromatography, Digestions, Purification

Control Regions for pGEX Vectors

From our library of Protocols, Sigma-Aldrich presents Control Regions for pGEX Vectors

Purification of Membrane Proteins

Extracted from Purifying Challenging Proteins - Principles and Methods, GE Healthcare, 2007
Keywords: Adsorption, Buffers, Cell culture, Cell disruption, Characterizations, Chromatography, Degradations, Detergents, Filtration, Immobilization, Ion Exchange, Ligands, PAGE, Sample preparations, Separation, Sonication, Tagged proteins

Removal of Thrombin and Factor Xa Using HiTrap Benzamidine FF (High Sub)

To protect the fusion protein from proteolytic degradation prior to enzymatic cleavage with PreScission Protease, thrombin, or Factor Xa, it may be necessary to remove proteases from the sample. Addi...
Keywords: Absorption, Cell culture, Chromatography, Degradations, PAGE, Purification

Troubleshooting Strategies in Purification of GST-tagged Proteins

This troubleshooting guide addresses the common problems associated with the majority of purification methods using the different Glutathione Sepharose media.
Keywords: Buffers, Cell disruption, Centrifugation, Degradations, Detergents, Electrophoresis, Gene expression, Ion Exchange, Precipitation, Purification, Sonication, Tagged proteins, Western blot

Troubleshooting in pGEX Expression Vectors

From our library of Protocols, Sigma-Aldrich presents Troubleshooting in pGEX Expression Vectors
Keywords: Cell disruption, Cloning, Gene expression, Sequencing

Troubleshooting of Cleavage Methods

The troubleshooting guide below addresses problems common to the majority of cleavage methods as well as problems specific to a particular method. In the latter case, the relevant method is indicated.
Keywords: Cloning, Electrophoresis, Purification, Tagged proteins, Western blot

pGEX Vectors

GST-tagged proteins are constructed by inserting a gene or gene fragment into the MCS of one of the 13 pGEX vectors. Expression is under the control of the tac promoter, which is induced by the lacto...
Keywords: Catalysis, Cloning, Gene expression, Immobilization, Purification, Tagged proteins

Peer-Reviewed Papers
15

References

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