EMAIL THIS PAGE TO A FRIEND
GE27-0849-01

Factor Xa

GE Healthcare, 27-0849-01, pack of 400 units

Purchase

Properties

shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging   pack of 400 units
mfr. no.   GE Healthcare, 27-0849-01
shipped in   dry ice
storage temp.   −20°C

Description

General description

The Glutathione S-transferase (GST) Gene Fusion System from GE Healthcare is a versatile system for the expression, purification, and detection of GST-tagged proteins produced in E. coli.

Application

Factor Xa, purified from bovine plasma, is a protease used to digest fusion Proteins prepared from pGEX vectors containing the recognition sequence for factor Xa (pGEX-3X, pGEX-5X-1, pGEX-5X-2, and pGEX-5X-3). It specifically cleaves following the tetrapeptide Ile-Glu-Gly-Arg.

Features and Benefits

• Enables the site-specific cleavage of fusion Proteins containing an accessible Factor Xa protease recognition sequence.
• Can be used either following affinity purification or while fusion Proteins are bound to Glutathione Sepharose® 4B, Glutathione Sepharose® High Performance, or Glutathione Sepharose® 4 Fast Flow.

Analysis Note

www.gelifesciences.com.

Legal Information

Sepharose is a registered trademark of Cytiva

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Protocols

Cleavage and Purification of GST-Tagged Protein Bound to GSTrap

The protocol below is an example optimized for 8 mg of target protein. It is worth estimating how much target protein is applied to the column, as this allows one to minimize the amount of protease a...
Keywords: Affinity chromatography, Buffers, Cell disruption, Centrifugation, Chromatography, Digestions, Filtration, PAGE, Purification, Sonication, Tagged proteins

Cleavage and Purification of GST-Tagged Protein Bound to Glutathione Sepharose in Batch Mode

Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, and Glutathione Sepharose 4B can all be used for cleavage and purification of GST-tagged proteins in batch.
Keywords: Buffers, Cell disruption, Centrifugation, Chromatography, Purification, Tagged proteins

Cleavage and Purification of GST-Tagged Protein Eluted from GSTrap

The protocol below is an example optimized for 8 mg of target protein. It is worth estimating how much target protein is applied to the column, as this allows one to minimize the amount of protease a...
Keywords: Absorption, Buffers, Chromatography, Digestions, Purification

Control Regions for pGEX Vectors

From our library of Protocols, Sigma-Aldrich presents Control Regions for pGEX Vectors

Removal of Thrombin and Factor Xa Using HiTrap Benzamidine FF (High Sub)

To protect the fusion protein from proteolytic degradation prior to enzymatic cleavage with PreScission Protease, thrombin, or Factor Xa, it may be necessary to remove proteases from the sample. Addi...
Keywords: Absorption, Cell culture, Chromatography, Degradations, PAGE, Purification

Troubleshooting Strategies in Purification of GST-tagged Proteins

This troubleshooting guide addresses the common problems associated with the majority of purification methods using the different Glutathione Sepharose media.
Keywords: Buffers, Cell disruption, Centrifugation, Degradations, Detergents, Electrophoresis, Gene expression, Ion Exchange, Precipitation, Purification, Sonication, Tagged proteins, Western blot

Troubleshooting in pGEX Expression Vectors

From our library of Protocols, Sigma-Aldrich presents Troubleshooting in pGEX Expression Vectors
Keywords: Cell disruption, Cloning, Gene expression, Sequencing

Troubleshooting of Cleavage Methods

The troubleshooting guide below addresses problems common to the majority of cleavage methods as well as problems specific to a particular method. In the latter case, the relevant method is indicated.
Keywords: Cloning, Electrophoresis, Purification, Tagged proteins, Western blot

pGEX Vectors

GST-tagged proteins are constructed by inserting a gene or gene fragment into the MCS of one of the 13 pGEX vectors. Expression is under the control of the tac promoter, which is induced by the lacto...
Keywords: Catalysis, Cloning, Gene expression, Immobilization, Purification, Tagged proteins

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?