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GE27-4710-01

Anti-His Antibody

GE Healthcare, 27-4710-01

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Properties

Related Categories Alphabetical Index, Antibodies, HF-HI, Primary Antibodies
conjugate   unconjugated
shipped in   dry ice
storage temp.   −20°C
packaging   pkg of 170 μL
antibody product type   primary antibodies
shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
mfr. no.   GE Healthcare, 27-4710-01

Description

General description

Anti-His Antibody is an IgG2 subclass of monoclonal antibody directed against six histidine residues, which are commonly used as an affinity tag for recombinant fusion proteins. The antibody is unconjugated to offer the flexibility of detection with a secondary antibody conjugated with either horseradish peroxidase or alkaline phosphatase. The antibody provides high sensitivity with low background. Anti-His Antibody is quality tested to detect a GST-(histidine)6-tagged protein at levels of » 50 ng in ELISA and 100 ng in Western blots.

Application

Western Blots and ELISA

Features and Benefits

• For the immunodetection of histidine-tagged recombinant proteins in Western blots and ELISAs
• Produced from a monoclonal cell line cultivated in mice and provided as clarified ascites fluid
• Functionally tested for detecting a test histidine-tagged recombinant protein in Western blots and ELISA
• Provides sufficient monoclonal antibody to perform 25 Western blots, each containing 20 mL antibody solution

Analysis Note

To view the Certificate of Analysis for this product, please visit www.gelifesciences.com.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

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