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GE28-4013-53

His Spintrap

GE Healthcare, 28-4013-53

  •  NACRES NA.32

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Properties

Related Categories Affinity Chromatography, GE Healthcare Life Sciences Protein Sample Preparation Formats, IMAC Matrices, Molecular Biology, Native Protein Sample Preparation,
shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
mfr. no.   GE Healthcare, 28-4013-53
packaging   pkg of 50 x 100 μL
bed volume   100 μL
matrix   highly cross-linked 6% agarose
avg. part. size   34 μm
cleaning   2 - 14 (Ni2+-stripped medium.)
working range   3 - 12 (Ni2+-stripped medium.)
capacity   ≥40 mg binding capacity (histidine-tagged protein/ml medium)(Protein binding capacity is protein-to-protein dependent.)
  ≥40 mg binding capacity (histidine-tagged protein/ml medium)(Protein binding capacity is protein-to-protein dependent.)

Description

General description

His SpinTrap is a prepacked, single-use spin column for purifying histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC)

* See the section for licensing information in our terms and conditions.

Purification of unclarified samples on His SpinTrap columns minimizes loss of target protein caused by manual operations such as sample precentrifugation, transfer to centrifugation tubes, and collecting supernatant. In addition, loading unclarified sample directly to His SpinTrap columns reduces sample preparation time, which minimizes degradation and oxidation of sensitive target proteins.

His SpinTrap columns are used together with a standard microcentrifuge and one purification run takes approximately 10 min. For optimal performance, use His SpinTrap together with buffers prepared using His Buffer Kit.

The columns are prepacked with Ni Sepharose® High Performance, which is precharged with nickel ions for capture of histidine-tagged proteins. Leakage of nickel ions from the Ni Sepharose® High Performance medium is negligible.

Features and Benefits

• For simple, small-scale purification of histidine-tagged* proteins and rapid expression screening.
• Ni Sepharose® High Performance in prepacked spin columns.
• Yields up to 750 μg pure protein per column.
• Allows direct purification of both clarified and unclarified cell lysates.

Storage and Stability

Store at 4 to 30 °C (0.15% Kathon CG)

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.gelifesciences.com.

Legal Information

Sepharose is a registered trademark of Cytiva

SpinTrap is a trademark of Cytiva

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Protocols

Characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and Uncharged IMAC Sepharose Products

Appendix 1, Extracted from Affinity Chromatography Vol. 2: Tagged Proteins, GE Healthcare, 2016  
Keywords: Affinity chromatography, Buffers, Cell culture, Centrifugation, Chromatography, Detergents, Immobilization, Precipitation, Purification, Tagged proteins

Desalting/Buffer Exchange and Concentration for Affinity Chromatography of Tagged Proteins

Desalting at laboratory scale is a well-proven, simple, and very fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired bu...
Keywords: Absorption, Addition reactions, Affinity chromatography, Buffers, Centrifugation, Chromatography, Detergents, Dialysis, Fractionation, Ion Exchange, PAGE, Precipitation, Sample preparations, Separation, Size-exclusion chromatography, Tagged proteins

Manual and Automated Purification

Chapter 2, Extracted from Affinity Chromatography Vol. 2: Tagged Proteins, GE Healthcare, 2016
Keywords: Affinity chromatography, Centrifugation, Chromatography, Gene expression, Tagged proteins

Minipreps using His SpinTrap and His SpinTrap Kit

His SpinTrap™ and His SpinTrap™ Kit are designed for efficient minipreps (small-scale purification) of histidine-tagged proteins directly from clarified or unclarified cell lysates. The columns may also ...
Keywords: Affinity chromatography, Buffers, Cell culture, Cell disruption, Centrifugation, Chromatography, Degradations, Gene expression, Homogenization, PAGE, Sample preparations, Sonication, Tagged proteins

Optimizing Purification of Histidine-Tagged Proteins

Chapter 4, Extracted from Affinity Chromatography Vol. 2: Tagged Proteins, GE Healthcare, 2016
Keywords: Affinity chromatography, Buffers, Cell disruption, Chromatography, Immobilization, Ion Exchange, PAGE, Purification, Separation, Sequencing, Size-exclusion chromatography, Tagged proteins

Purification of Histidine-Tagged Recombinant Proteins Using Ni Sepharose High Performance

Ni Sepharose High Performance consists of highly cross-linked 6% agarose beads (34 µm) to which a chelating group has been immobilized and subsequently charged with Ni2+ ions. The chromatography medi...
Keywords: Affinity chromatography, Buffers, Cell disruption, Chromatography, Filtration, Homogenization, Immobilization, Purification, Sample preparations, Sonication, Tagged proteins

Troubleshooting Guide for Affinity Chromatography of Tagged Proteins

The troubleshooting guide below addresses problems common to the majority of purification products discussed in this chapter, as well as problems specific to a particular method. In the latter case, th...
Keywords: Addition reactions, Affinity chromatography, Buffers, Cell culture, Cell disruption, Centrifugation, Chromatography, Detergents, Fermentation, PAGE, Precipitation, Protein biosynthesis, Size-exclusion chromatography, Sonication, Tagged proteins, Western blot

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