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GE28-4082-53

HiTrap® MabSelect

Cytiva, 28-4082-53, pack of 5 × 1 mL

  •  NACRES NA.56

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Properties

Related Categories Analytical/Chromatography, Antibodies, Antibody Purification and Characterization, GE Healthcare Life Sciences Protein Sample Preparation Formats, HiTrap & PD-10 Cartridges,
shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging   pack of 5 × 1 mL
mfr. no.   Cytiva, 28-4082-53
parameter   <4 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
  42 psi
bed size   7 mm × 25 mm
bed volume   1 mL
column I.D.   7 mm
matrix   highly cross-linked agarose
avg. part. size   85 μm (d₅₀v is the median particle size of the cumulative volume distribution)
cleaning   2 - 12 (pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)
working range   3 - 10
capacity   ≥30 mg binding capacity (humanlgG/ml at 2.4 min residence time)(Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 500 cm/h in a column with a bed height of 20 cm.)
suitability   suitable for bioprocess medium
storage temp.   2-8°C

Description

General description

HiTrap® MabSelect columns are prepacked with MabSelect, a Bioprocess medium for capuring of Mabs from large sample volumes.

Application

Lab Purification using the MabSelect Protein A tag for capture of antibodies.

Features and Benefits

• HiTrap® columns prepacked with MabSelect, a Bioprocess medium for capuring of Mabs from large sample volumes. • The specificity of binding to the Fc region of IgG is similar to that of native protein A, which allows one-step purification.
• Oriented coupling of recombinant Protein A to the matrix via an engineered C-terminal cysteine enhances IgG binding capacity.
• Fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.gelifesciences.com.

Legal Information

HiTrap is a registered trademark of Cytiva

MabSelect is a trademark of Cytiva

Safety & Documentation

Safety Information

RIDADR 
UN1170 - class 3 - PG 3 - Ethanol, solution

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Column Packing and Preparation for Affinity Chromatography of Antibodies

Appendix 5, Extracted from Affinity Chromatography Vol. 1: Antibodies, GE Healthcare, 2016  
Keywords: Affinity chromatography, Antimicrobials, Chromatography, Detergents, Gene expression, Separation, Solvents

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer ...
Keywords: Absorption, Affinity chromatography, Buffers, Centrifugation, Chromatography, Detergents, Dialysis, Electrophoresis, Fractionation, Gel electrophoresis, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, PAGE, Precipitation, Sample preparations, Separation, Size-exclusion chromatography, Titrations

Performing a Purification of IgG Antibodies with HiTrap® MabSelect and HiTrap® MabSelect Xtra

This section describes a general procedure for purification of MAbs using HiTrap® MabSelect and HiTrap® MabSelect Xtra prepacked columns.
Keywords: Affinity chromatography, Buffers, Chromatography, PAGE, Sample preparations, Size-exclusion chromatography

Selection Guide for Polymeric Resins in Chromatography & Purification

Sigma-Aldrich carries a vast array of resins, from leading manufacturers; such as, Dow Chemical, Rohm & Haas, Mitsubishi, and Lanxess. Our product line includes adsorbent, ion-exchange (anion / catio...
Keywords: Adsorption, Antibiotics, Combinatorial synthesis, Detergents, Fermentation, Filtration, Hormones, Ion Exchange, Ion exchange resins, Pharmaceutical, Purification, Separation

Protocols

Affinity Chromatography Troubleshooting

This section focuses on practical problems that may occur when running a chromatography column. The diagrams below give an indication of how a chromatogram may deviate from the ideal during affnity p...
Keywords: Adsorption, Affinity chromatography, Buffers, Chromatography, Detergents, Digestions, Fractionation, Ligands, PAGE, Precipitation, Purification, Sample preparations, Separation, Solvents

Buffer Exchange and Desalting for Affinity Chromatography

Dialysis is frequently mentioned in the literature as a technique to remove salt or other small molecules and to exchange the buffer composition of a sample. However, dialysis is generally a very slo...
Keywords: Absorption, Affinity chromatography, Buffers, Chromatography, Dialysis, Separation

Column Packing and Preparation for Affinity Chromatography

Appendix 3,  Extracted from Affinity Chromatography Principles and Methods, GE Healthcare, 2007
Keywords: Affinity chromatography, Antimicrobials, Buffers, Chromatography, Separation

Converting From Linear Fow (cm/hour) to Volumetric Flow Rates (ml/min) and Vice Versa

Appendix 4 extracted from Affinity Chromatography Principles and Methods, GE Healthcare, 2007
Keywords: Affinity chromatography, Chromatography

Performing a Separation with GE Healthcare Products Based on Protein A

Protein A is derived from a strain of Staphylococcus aureus and contains five regions that bind to the Fc region of IgG. As an affinity ligand, protein A is coupled to Sepharose so that these regions a...
Keywords: Affinity chromatography, Buffers, Cell culture, Chromatography, Ligands, PAGE, Purification

Sample Preparation for Affinity Chromatography

Appendix 1 extracted from Affinity Chromatography Principles and Methods, GE Healthcare, 2007
Keywords: Adsorption, Affinity chromatography, Buffers, Centrifugation, Chromatography, Detergents, Filtration, Nucleic acid denaturation, PAGE, Precipitation, Sample preparations

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