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  • GE28-4082-60 - HiTrap® MabSelect Xtra


HiTrap® MabSelect Xtra

Cytiva, 28-4082-60, pack of 1 × 5 mL

  •  NACRES NA.56



Related Categories Antibodies, Antibody Purification and Characterization, GE Healthcare Life Sciences Protein Sample Preparation Formats, HiTrap System/syringe, MabSelect mAb Purification,
shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging   pack of 1 × 5 mL
mfr. no.   Cytiva, 28-4082-60
parameter   <20 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
  42 psi
bed size   16 mm × 25 mm
bed volume   5 mL
column I.D.   16 mm
matrix   highly cross-linked agarose
avg. part. size   75 (d₅₀v is the median particle size of the cumulative volume distribution)
cleaning   2 - 12 (pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)
working range   3 - 10 (pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)
capacity   ≥30 mg binding capacity (humanlgG/ml at 2.4 min residence time)(Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 500 cm/h in a column with a bed height of 20 cm.)
suitability   suitable for bioprocess medium
storage temp.   2-8°C


Analysis Note


Legal Information

HiTrap is a registered trademark of Cytiva

MabSelect is a trademark of Cytiva

Safety & Documentation

Safety Information

UN1170 - class 3 - PG 3 - Ethanol, solution


Certificate of Analysis (COA)

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Protocols & Articles


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Appendix 5, Extracted from Affinity Chromatography Vol. 1: Antibodies, GE Healthcare, 2016  
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Performing a Purification of IgG Antibodies with HiTrap® MabSelect and HiTrap® MabSelect Xtra

This section describes a general procedure for purification of MAbs using HiTrap® MabSelect and HiTrap® MabSelect Xtra prepacked columns.
Keywords: Affinity chromatography, Buffers, Chromatography, PAGE, Sample preparations, Size-exclusion chromatography

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