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GE28-4083-47

Ab Spintrap

GE Healthcare, 28-4083-47

  •  NACRES NA.25

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Properties

Related Categories GE Healthcare Life Sciences Protein Sample Preparation Formats, Molecular Biology, Native Protein Sample Preparation, Proteomics, SpinTrap Microcentrifuge More...
shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging   pkg of 50 × 100 μL
mfr. no.   GE Healthcare, 28-4083-47
bed volume   100 μL
matrix   highly cross-linked 6% agarose
avg. part. size   34 μm
cleaning   2 - 9 (pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)
working range   3 - 9
capacity   >1 mg binding capacity (lgG/column)(The binding capacity has been determined with human polyclonal IgG.)
  >1 mg binding capacity(IgG/column)
storage temp.   2-8°C

Description

General description

Ab SpinTrap are prepacked, single-use spin columns for rapid purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants. Designed for small-scale purification of multiple samples in parallel.

Application

Ab SpinTrap is designed for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants. In addition, a protocol for immunoprecipitation is included.


The spin columns are also suitable for use in antibody screening experiments.


Ab SpinTrap columns are used together with a standard microcentrifuge and one purification run is performed in less than 20 min.


Ab SpinTrap contains Protein G Sepharose® High Performance, which has high Protein binding capacity and is compatible with all commonly buffers used in antibody purification with immobilized Protein G media.


For optimal performance in antibody purification, use Ab SpinTrap together with buffers prepared using Ab Buffer Kit.

Features and Benefits

• Rapid and convenient small-scale purification of IgG.
• Protein G Sepharose® High Performance in prepacked spin columns.
• No filtration or centrifugation of different crude samples necessary for antibody purification.
• Classic and crosslink protocols for Protein enrichment (immunoprecipitation).
• Elution conditions formatted for both LC-MS and electrophoresis analysis.
• Elution conditions formatted for both LC-MS and electrophoresis analysis.

Analysis Note

www.gelifesciences.com.

Legal Information

Sepharose is a registered trademark of Cytiva

SpinTrap is a trademark of Cytiva

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer ...
Keywords: Absorption, Affinity chromatography, Buffers, Centrifugation, Chromatography, Detergents, Dialysis, Electrophoresis, Fractionation, Gel electrophoresis, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, PAGE, Precipitation, Sample preparations, Separation, Size-exclusion chromatography, Titrations

Performing a Purification of IgG Antibodies with Protein G HP SpinTrap™/Ab SpinTrap™

Protein G HP SpinTrap™ are prepacked, single-use spin columns for protein enrichment of target antigens from antibody-antigen complexes of monoclonal and polyclonal antibodies from unclarified serum a...
Keywords: Affinity chromatography, Buffers, Cell culture, Centrifugation, Chromatography, Immunoprecipitation, Ligands, PAGE, Sample preparations

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