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GE28-9030-59

Ab Buffer Kit

GE Healthcare, 28-9030-59, pack of 1 kit

  •  NACRES NA.25

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Properties

shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging   pack of 1 kit
mfr. no.   GE Healthcare, 28-9030-59
storage temp.   2-8°C

Description

General description

Ab Buffer Kit contains carefully prepared buffer concentrates for binding, washing, and elution of IgG according to recommended protocols. The kit also includes neutralizing buffer. Includes: 1 × 50 ml Binding buffer 10× stock solution, 1 × 15 ml Elution buffer 10× stock solution, and 1 × 25 ml Neutralizing buffer.

Analysis Note

www.gelifesciences.com.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer ...
Keywords: Absorption, Affinity chromatography, Buffers, Centrifugation, Chromatography, Detergents, Dialysis, Electrophoresis, Fractionation, Gel electrophoresis, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, PAGE, Precipitation, Sample preparations, Separation, Size-exclusion chromatography, Titrations

Performing a Purification of IgG Antibodies with HiTrap® Protein G HP Columns

HiTrap® Protein G HP (Figure 3.10) is a convenient, ready-to-use column prepacked with Protein G Sepharose® High Performance. The columns are available in 1 ml and 5 ml sizes. In common with all HiTr...
Keywords: Affinity chromatography, Buffers, Cell culture, Chromatography, Immunodiffusion, Ligands, PAGE, Sample preparations

Performing a Purification of IgG Antibodies with Protein G GraviTrap™

Protein G GraviTrap™ are gravity-flow columns prepacked with 1 ml of Protein G Sepharose® 4 Fast Flow. The columns are designed for fast and efficient manual purification of monoclonal and polyclonal an...
Keywords: Affinity chromatography, Buffers, Cell culture, Chromatography, Ligands, Sample preparations

Performing a Purification of IgG Antibodies with Protein G HP MultiTrap™

Protein G HP MultiTrap™ are 96-well plates prepacked with Protein G Sepharose® High Performance. Protein G HP MultiTrap™ is a versatile tool for screening of different proteins and for preparation of...
Keywords: Affinity chromatography, Buffers, Chromatography, Immunoprecipitation, Sample preparations

Performing a Purification of IgG Antibodies with Protein G HP SpinTrap™/Ab SpinTrap™

Protein G HP SpinTrap™ are prepacked, single-use spin columns for protein enrichment of target antigens from antibody-antigen complexes of monoclonal and polyclonal antibodies from unclarified serum a...
Keywords: Affinity chromatography, Buffers, Cell culture, Centrifugation, Chromatography, Immunoprecipitation, Ligands, PAGE, Sample preparations

Purification using protein A chromatography media

Protein A is derived from a strain of Staphylococcus aureus and contains five regions that bind to the Fc region of IgG. As an affinity ligand, protein A is coupled to Sepharose® so that these region...
Keywords: Affinity chromatography, Buffers, Chromatography, Fermentation, Immunoprecipitation, Ligands, Purification, Size-exclusion chromatography

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