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GE28-9321-71

HIS Spintrap Kit

GE Healthcare, 28-9321-71, pack of 1 kit

  •  NACRES NA.56

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Properties

Related Categories GE Healthcare Life Sciences Protein Sample Preparation Formats, Molecular Biology, Native Protein Sample Preparation, Proteomics, SpinTrap Microcentrifuge More...
shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging   pack of 1 kit
mfr. no.   GE Healthcare, 28-9321-71
matrix   highly cross-linked 6% agarose
avg. part. size   34 μm
cleaning   2 - 14 (Ni2+-stripped medium.)
working range   3 - 12 (Ni2+-stripped medium.)
capacity   ≥40 mg binding capacity (histidine-tagged protein/ml medium)(Protein binding capacity is protein-to-protein dependent.)
storage temp.   2-8°C

Description

Components

The kit contains:

1 pack - GE28-4013-53 - His Spin Trap
1 pack - GE11-0034-00 - His Buffer Kit

Analysis Note

www.gelifesciences.com.

Legal Information

SpinTrap is a trademark of Cytiva

Kit component also available separately

Description

Product #

Add to Cart

His Buffer Kit, GE Healthcare, 11-0034-00 SDS GE11-0034-00
His Spintrap, GE Healthcare, 28-4013-53 SDS GE28-4013-53
Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Protocols

Characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and Uncharged IMAC Sepharose Products

Appendix 1, Extracted from Affinity Chromatography Vol. 2: Tagged Proteins, GE Healthcare, 2016  
Keywords: Affinity chromatography, Buffers, Cell culture, Centrifugation, Chromatography, Detergents, Immobilization, Precipitation, Purification, Tagged proteins

Minipreps using His SpinTrap and His SpinTrap Kit

His SpinTrap™ and His SpinTrap™ Kit are designed for efficient minipreps (small-scale purification) of histidine-tagged proteins directly from clarified or unclarified cell lysates. The columns may also ...
Keywords: Affinity chromatography, Buffers, Cell culture, Cell disruption, Centrifugation, Chromatography, Degradations, Gene expression, Homogenization, PAGE, Sample preparations, Sonication, Tagged proteins

Optimizing Purification of Histidine-Tagged Proteins

Chapter 4, Extracted from Affinity Chromatography Vol. 2: Tagged Proteins, GE Healthcare, 2016
Keywords: Affinity chromatography, Buffers, Cell disruption, Chromatography, Immobilization, Ion Exchange, PAGE, Purification, Separation, Sequencing, Size-exclusion chromatography, Tagged proteins

Purification of Histidine-Tagged Recombinant Proteins Using Ni Sepharose High Performance

Ni Sepharose High Performance consists of highly cross-linked 6% agarose beads (34 µm) to which a chelating group has been immobilized and subsequently charged with Ni2+ ions. The chromatography medi...
Keywords: Affinity chromatography, Buffers, Cell disruption, Chromatography, Filtration, Homogenization, Immobilization, Purification, Sample preparations, Sonication, Tagged proteins

Troubleshooting Guide for Affinity Chromatography of Tagged Proteins

The troubleshooting guide below addresses problems common to the majority of purification products discussed in this chapter, as well as problems specific to a particular method. In the latter case, th...
Keywords: Addition reactions, Affinity chromatography, Buffers, Cell culture, Cell disruption, Centrifugation, Chromatography, Detergents, Fermentation, PAGE, Precipitation, Protein biosynthesis, Size-exclusion chromatography, Sonication, Tagged proteins, Western blot

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