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GE28-9355-97

Dextrin Sepharose® High Performance

GE Healthcare, 28-9355-97, pack of 25 mL

  •  NACRES NA.56

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Properties

shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging   pack of 25 mL
mfr. no.   GE Healthcare, 28-9355-97
matrix   highly cross-linked 6% agarose
avg. part. size   34 μm
cleaning in place   2 - 13
working range   >7
capacity   ~7 mg binding capacity (MBP- ΔSal/ml medium Approx. 16 mg MBP- ßGal/ml medium)(Binding capacity is protein dependent. Dynamic binding capacity was tested with MBP-?Sal (MBP2*-paramyosin-?Sal, Mr approx. 70 000, multimer in solution), and MBP-ßGal (MBP2*-beta-galactosidase, Mr approx. 158 000, multimer in solution).)
storage temp.   2-8°C

Description

General description

Dextrin Sepharose® High Perfromance is an affinity media for purification of proteins tagged with Maltose Binding protein (MBP).

Application

Dextrin Sepharose® High Performance and provide convenient affinity purification of recombinant Proteins tagged with maltose binding Protein (MBP). This robust and stable Sepharose® High Performance based medium enables elution of target Protein in narrow peaks, which minimizes the need for further concentration steps.

Features and Benefits

• Dextrin Sepharose® High Performance is a robust, high resolution medium based on the 34 μm Sepharose® High Performance matrix
• The small, evenly sized beads ensure that MBP-tagged Proteins elute in narrow peaks, thus minimizing the need for further concentration steps
• Physiological conditions and mild elution preserve target Protein activity
• Dextrin Sepharose® High Performance tolerates all commonly used aqueous buffers and is easily regenerated using 0.5 M NaOH allowing the same column to be used for repeated purifications

Analysis Note

www.gelifesciences.com.

Legal Information

Sepharose is a registered trademark of Cytiva

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Protocols

Characteristics of Dextrin Sepharose High Performance Products

Appendix 3, Extracted from Affinity Chromatography Vol. 2: Tagged Proteins, GE Healthcare, 2016  
Keywords: Affinity chromatography, Buffers, Chromatography, Ligands, Tagged proteins

Column Cleaning for Ion Exchange Chromatography and Chromatofocusing

Appendix 10, extracted from Ion Exchange Chromatography & Chromatofocusing, GE Healthcare, 2007
Keywords: Buffers, Chromatography, Detergents, Ion Exchange, Separation, Solvents

Column Packing and Preparation

Appendix 6, Extracted from Affinity Chromatography Vol. 2: Tagged Proteins, GE Healthcare, 2016  
Keywords: Affinity chromatography, Antimicrobials, Chromatography, Gene expression, Separation, Tagged proteins

Desalting/Buffer Exchange and Concentration for Affinity Chromatography of Tagged Proteins

Desalting at laboratory scale is a well-proven, simple, and very fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired bu...
Keywords: Absorption, Addition reactions, Affinity chromatography, Buffers, Centrifugation, Chromatography, Detergents, Dialysis, Fractionation, Ion Exchange, PAGE, Precipitation, Sample preparations, Separation, Size-exclusion chromatography, Tagged proteins

Purification using Dextrin Sepharose High Performance

Dextrin Sepharose® High Performance is a robust, high-resolution chromatography medium based on the 34 µm Sepharose® High Performance. The small, evenly sized beads ensure that MBP-tagged proteins el...
Keywords: Affinity chromatography, Buffers, Chromatography, Homogenization, Sample preparations, Sonication, Tagged proteins

Purification using MBPTrap HP Columns

MBPTrap HP 1 ml and 5 ml columns (Fig 6.2) are made of biocompatible polypropylene that does not interact with biomolecules. Prepacked MBPTrap HP columns provide fast, simple, and easy separations in...
Keywords: Affinity chromatography, Buffers, Cell disruption, Chromatography, Homogenization, PAGE, Sample preparations, Size-exclusion chromatography, Sonication, Tagged proteins

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