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GE28-9365-51

Hisprep Fast Flow 16/10

GE Healthcare, 28-9365-51

  •  NACRES NA.56

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Properties

Related Categories Affinity Chromatography, Heparin, IMAC Matrices, Molecular Biology, Protein Chromatography,
shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging   pkg of 1 ea
mfr. no.   GE Healthcare, 28-9365-51
parameter   <10 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
  1.5 bar (22 psi) (Over the Packed Bed During Operation)
  22 psi
bed size   16 mm × 100 mm
bed volume   20 mL
column I.D.   16 mm
matrix   6% cross-linked agarose
particle size   45-165 μm
average diameter   90 μm
cleaning   2 - 14 (Ni2+-stripped medium.)
working range   3 - 12 (Ni2+-stripped medium.)
capacity   ~40 mg binding capacity(histidine-tagged protein)
suitability   suitable for bioprocess medium

Description

General description

HisPrep FF 16/10 is a ready-to-use column, prepacked with precharged Ni Sepharose® 6 Fast Flow. The column is an excellent choice for preparative, scale-up purification of histidine-tagged recombinant proteins.

HisPrep FF 16/10 columns are prepacked with Ni Sepharose® 6 Fast Flow for scale-up purification of histidine-tagged proteins. Ni Sepharose® 6 Fast Flow consists of 90 ?m beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. This chelating ligand is charged with Ni2+ ions, the first-choice metal ion for purifying most histidine-tagged proteins. The negligible leakage of Ni2+ ions ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications.

Features and Benefits

• Optimized for convenient scale-up purification of histidine-tagged proteins.
• Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.
• Negligible leakage of Ni2+.
• High binding capacity, approx. 40 mg/mL medium.
• Convenient and time-saving 20 mL prepacked HiPrep format.

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.gelifesciences.com.

Legal Information

HiPrep is a trademark of Cytiva

HisPrep is a trademark of Cytiva

Sepharose is a registered trademark of Cytiva

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Converting from Flow Velocity to Volumetric Flow Rates

Appendix 7, Extracted from Affinity Chromatography Vol. 1: Antibodies, GE Healthcare, 2016  
Keywords: Affinity chromatography, Chromatography

Protocols

Characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and Uncharged IMAC Sepharose Products

Appendix 1, Extracted from Affinity Chromatography Vol. 2: Tagged Proteins, GE Healthcare, 2016  
Keywords: Affinity chromatography, Buffers, Cell culture, Centrifugation, Chromatography, Detergents, Immobilization, Precipitation, Purification, Tagged proteins

Column Packing and Preparation for Affinity Chromatography with Specific Groups of Biomolecules

Appendix 3, extracted from Affinity Chromatography Vol. 3: Specific Groups of Biomolecules, GE Healthcare, 2014
Keywords: Antimicrobials, Chromatography, Gene expression, Separation

Desalting/Buffer Exchange and Concentration for Affinity Chromatography of Tagged Proteins

Desalting at laboratory scale is a well-proven, simple, and very fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired bu...
Keywords: Absorption, Addition reactions, Affinity chromatography, Buffers, Centrifugation, Chromatography, Detergents, Dialysis, Fractionation, Ion Exchange, PAGE, Precipitation, Sample preparations, Separation, Size-exclusion chromatography, Tagged proteins

Manual and Automated Purification

Chapter 2, Extracted from Affinity Chromatography Vol. 2: Tagged Proteins, GE Healthcare, 2016
Keywords: Affinity chromatography, Centrifugation, Chromatography, Gene expression, Tagged proteins

Performing a purification and on-column refolding of an insoluble histidine-tagged protein from a 100 ml E. coli culture using HisTrap FF 1 ml with ÄKTAprime plus

This procedure uses a HisTrap FF 1 ml column but can also be used with a HisTrap HP 1 ml or a HisTrap FF crude 1 ml column. The procedure uses ÄKTAprime plus but can also be run on other ÄKTA systems...
Keywords: Buffers, Chromatography, Fractionation, Sonication, Tagged proteins

Preparative Purification Using HisPrep™ FF 16/10

HisPrep™ FF 16/10 columns are specially designed 20 ml HiPrep columns, ready to use for easy, one-step preparative purification of histidine-tagged proteins. Prepacked with Ni Sepharose® 6 Fast Flow, ...
Keywords: Affinity chromatography, Buffers, Chromatography, Purification, Tagged proteins

Purification or Removal of Proteins and Peptides with Exposed Amino Acids: His, Cys, Trp, and/or with Affinity for Metal Ions

Extracted from Affinity Chromatography Vol. 3: Specific Groups of Biomolecules, GE Healthcare, 2014
Keywords: Adsorption, Buffers, Chromatography, Detergents, Immobilization, Ion Exchange, Ligands, Phase transitions, Precipitation, Reductions, Separation, Tagged proteins

Purification Using HisTrap HP and HisTrap FF

HisTrap HP and HisTrap FF are 1 ml and 5 ml HiTrap columns packed with Ni Sepharose High Performance or Ni Sepharose 6 Fast Flow, respectively. Sample application, washing,  and elution can be perfor...
Keywords: Affinity chromatography, Buffers, Chromatography, PAGE, Purification, Size-exclusion chromatography, Tagged proteins

Purification of Histidine-Tagged Recombinant Proteins Using Ni Sepharose 6 Fast Flow

Ni Sepharose 6 Fast Flow consists of 90 µm beads of highly cross-linked agarose, to which a chelating ligand has been immobilized and subsequently charged with Ni2+ ions. The ligand density of Ni Sep...
Keywords: Affinity chromatography, Buffers, Cell disruption, Chromatography, Filtration, Homogenization, Immobilization, Ligands, Sample preparations, Sonication, Tagged proteins

Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules

Appendix 1, extracted from Affinity Chromatography Vol. 3: Specific Groups of Biomolecules, GE Healthcare, 2014
Keywords: Absorption, Adsorption, Buffers, Cell culture, Centrifugation, Chromatography, Detergents, Dialysis, Electrophoresis, Filtration, Indicators, Nucleic acid denaturation, Precipitation, Sample preparations, Separation, Tagged proteins

Troubleshooting Guide for Affinity Chromatography of Tagged Proteins

The troubleshooting guide below addresses problems common to the majority of purification products discussed in this chapter, as well as problems specific to a particular method. In the latter case, th...
Keywords: Addition reactions, Affinity chromatography, Buffers, Cell culture, Cell disruption, Centrifugation, Chromatography, Detergents, Fermentation, PAGE, Precipitation, Protein biosynthesis, Size-exclusion chromatography, Sonication, Tagged proteins, Western blot

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