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GE28-9464-89

HiTrap® rProtein A Fast Flow

GE Healthcare, 28-9464-89, pack of 100 × 1 mL

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Properties

Related Categories GE Healthcare Life Sciences Protein Sample Preparation Formats, HiTrap System/syringe, Molecular Biology, Native Protein Sample Preparation, Proteomics More...
shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging   pack of 100 × 1 mL
mfr. no.   GE Healthcare, 28-9464-89
parameter   <4 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
  42 psi
bed size   7 mm × 25 mm
bed volume   1 mL
column I.D.   7 mm
matrix   4% cross-linked agarose
particle size   60-165 μm
average diameter   90 μm (d50v)
cleaning   2 - 11 (pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)
working range   3 - 10
capacity   ~50 mg binding capacity (human lgG/ml medium)(Capacity of HiTrap rProtein A for some monoclonal antibodies. Running conditions: Binding buffer: 20 mM sodium phosphate (incl. 3 M NaCl for IgG), pH 7.0. Elution buffer: 0.1 M sodium citrate, pH 3.0.)
suitability   suitable for bioprocess medium
storage temp.   2-8°C

Description

Analysis Note

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Legal Information

HiTrap is a registered trademark of Cytiva

Safety & Documentation

Safety Information

Symbol 
GHS02  GHS02
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
UN1170 - class 3 - PG 3 - Ethanol, solution

Documents

Certificate of Analysis (COA)

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