• USA Home
  • GE28-9523-59 - GST Spintrap

EMAIL THIS PAGE TO A FRIEND
GE28-9523-59

GST Spintrap

GE Healthcare, 28-9523-59

  •  NACRES NA.56

Purchase

Properties

Related Categories GE Healthcare Life Sciences Protein Sample Preparation Formats, Molecular Biology, Native Protein Sample Preparation, Proteomics, SpinTrap Microcentrifuge More...
shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging   pkg of 50 ea
mfr. no.   GE Healthcare, 28-9523-59
bed volume   50 μL
matrix   4% agarose
particle size   45-165 μm
average diameter   90 μm
cleaning   4 - 13
working range   4 - 13
capacity   >25 mg binding capacity (horse liver GST/ml medium)(Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.)
  ≥500 μg binding capacity(recombinant glutathione S-transferase (rGST))

Description

Storage and Stability

Store at 4 to 30 °C (PBS and 0,05% Kathon CG/ICP Biocide)

Analysis Note

www.gelifesciences.com.

Legal Information

SpinTrap is a trademark of Cytiva

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Protocols

Characteristics of Glutathione Sepharose Products

Appendix 2, Extracted from Affinity Chromatography Vol. 2: Tagged Proteins, GE Healthcare, 2016  
Keywords: Affinity chromatography, Buffers, Centrifugation, Chromatography, Inductively coupled plasma, Ligands, Purification, Tagged proteins

Characteristics of Glutathione Sepharose and HiTrap Benzamidine FF (High Sub) Media and Columns

Glutathione Sepharose High Performance is recommended for high-resolution purification of GST-tagged proteins, providing sharp peaks and concentrated eluent. Glutathione Sepharose 4 Fast Flow is exce...
Keywords: Buffers, Centrifugation, Chromatography, Inductively coupled plasma, Ligands, Purification, Tagged proteins

Desalting/Buffer Exchange and Concentration for Affinity Chromatography of Tagged Proteins

Desalting at laboratory scale is a well-proven, simple, and very fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired bu...
Keywords: Absorption, Addition reactions, Affinity chromatography, Buffers, Centrifugation, Chromatography, Detergents, Dialysis, Fractionation, Ion Exchange, PAGE, Precipitation, Sample preparations, Separation, Size-exclusion chromatography, Tagged proteins

Manual and Automated Purification

Chapter 2, Extracted from Affinity Chromatography Vol. 2: Tagged Proteins, GE Healthcare, 2016
Keywords: Affinity chromatography, Centrifugation, Chromatography, Gene expression, Tagged proteins

Minipreps Using GST SpinTrap

GST SpinTrap™ columns are designed for rapid, small-scale purification of GST-tagged proteins using conditions of mild affinity purification. Greater than 90% purity can be achieved in a single step. Th...
Keywords: Addition reactions, Affinity chromatography, Buffers, Cell culture, Cell disruption, Centrifugation, Chromatography, Gene expression, Homogenization, Oxidations, Protein extraction, Sample preparations, Sonication, Tagged proteins

Pull-down assays

Extracted from Purifying Challenging Proteins - Principles and Methods, GE Healthcare, 2007
Keywords: Buffers, Cell disruption, Centrifugation, Chromatography, Cloning, Digestions, Gene expression, Genetic, Immobilization, Immunoprecipitation, Ligands, Mass spectrometry, PAGE, Precipitation

Purification Options for GST-tagged proteins using Glutathione Sepharose Products

Table 5.1 Purification options for GST-tagged proteins using Glutathione Sepharose Products
Keywords: Affinity chromatography, Chromatography, Purification, Tagged proteins

Purification Using GST SpinTrap

GST SpinTrap columns are designed for rapid, small-scale purification of GST-tagged proteins using mild conditions of affinity purification. More than 90% purity can be achieved in a single step. The...
Keywords: Addition reactions, Buffers, Cell culture, Cell disruption, Centrifugation, Gene expression, Homogenization, Oxidations, PAGE, Protein extraction, Purification, Sample preparations, Sonication, Tagged proteins

Removal of GST tag by enzymatic cleavage

Removal of the GST tag is often necessary to be able to perform functional or structural studies of the target protein. Tagged proteins containing a PreScission Protease, thrombin, or Factor Xa recog...
Keywords: Absorption, Affinity chromatography, Buffers, Cell disruption, Centrifugation, Chromatography, Digestions, PAGE, Size-exclusion chromatography, Sonication, Tagged proteins

Troubleshooting Purification Methods

The troubleshooting guide below addresses problems common to the majority of purification methods as well as problems specific to a particular method. In the latter case, the relevant method is indicat...
Keywords: Affinity chromatography, Buffers, Cell disruption, Centrifugation, Chromatography, Degradations, Detergents, Electrophoresis, Gene expression, Ion Exchange, Precipitation, Sonication, Tagged proteins, Western blot

Troubleshooting Strategies in Purification of GST-tagged Proteins

This troubleshooting guide addresses the common problems associated with the majority of purification methods using the different Glutathione Sepharose media.
Keywords: Buffers, Cell disruption, Centrifugation, Degradations, Detergents, Electrophoresis, Gene expression, Ion Exchange, Precipitation, Purification, Sonication, Tagged proteins, Western blot

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?