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GE28-9537-66

HiTrap® TALON® Crude

GE Healthcare, 28-9537-66, pack of 5 × 1 mL

  •  NACRES NA.56

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Properties

Related Categories GE Healthcare Life Sciences Protein Sample Preparation Formats, HiTrap System/syringe, Molecular Biology, Native Protein Sample Preparation, Proteomics More...
shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging   pack of 5 × 1 mL
mfr. no.   GE Healthcare, 28-9537-66
parameter   1 ml/min - 4 ml/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
  3 bar
column I.D.   7 mm
matrix   6% cross-linked agarose
particle size   60-160 μm
cleaning   2 - 14 (Co2+-stripped medium)
working range   3 - 12 (Co2+-stripped medium)
capacity   ≥20 mg binding capacity (histidine-tagged protein)(The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
storage temp.   2-8°C

Description

General description

HiTrap® Talon® crude 1 mL and 5 mL columns allow for rapid separations and easy scale-up with a minimum of sample preparation and equipment.

Application

Talon® Superflow is a cobalt-based IMAC medium offering different selectivity compared to nickel-charged media. Talon® Superflow and prepacked HiTrap® Talon® crude columns allow Protein purification under native or denaturing conditions and can be used with prokaryotic and eukaryotic expression systems.

HiTrap® Talon® crude columns allow for rapid separations and easy scale-up. Samples can be applied directly to the column after thorough cell disruption - without precentrifugation and filtration. The filters in the top and bottom of the column make it possible to directly load unclarified lysates on the column without causing back pressure problems or leakage of the Talon® Superflow beads. Prior to loading samples on HiTrap® Talon® crude columns, cell lysate should be prepared using methods such as sonication, homogenization, or freeze/thaw.

Features and Benefits

• Columns prepacked with cobalt-based chromatography media designed for purification of histidine-tagged recombinant Proteins by immobilized metal affinity chromatography (IMAC).
• Suitable for IMAC purification of histidine-tagged Proteins when Ni2+ is not the optimal choice of metal ion.
• Compatible with commonly used IMAC reagents and appropriate for purifying Proteins under native or denaturing conditions.
• Prepacked HiTrap® Talon® crude columns save time by allowing the application of unclarified samples directly, minimizing labor and degradation of Proteins.
• HiTrap® Talon® crude columns allow repeated runs without stripping, cleaning, or Co2+ recharging.

Analysis Note

www.gelifesciences.com.

Legal Information

HiTrap is a registered trademark of Cytiva

Talon is a registered trademark of Clontech

Safety & Documentation

Safety Information

Symbol 
GHS02  GHS02
Signal word 
Warning-Warning
Hazard statements 
RIDADR 
UN1170 - class 3 - PG 3 - Ethanol, solution
WGK Germany 
WGK 3
Flash Point(F) 
100.4 - 109.4 °F
Flash Point(C) 
38 - 43 °C

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Protocols

Characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and Uncharged IMAC Sepharose Products

Appendix 1, Extracted from Affinity Chromatography Vol. 2: Tagged Proteins, GE Healthcare, 2016  
Keywords: Affinity chromatography, Buffers, Cell culture, Centrifugation, Chromatography, Detergents, Immobilization, Precipitation, Purification, Tagged proteins

Desalting/Buffer Exchange and Concentration for Affinity Chromatography of Tagged Proteins

Desalting at laboratory scale is a well-proven, simple, and very fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired bu...
Keywords: Absorption, Addition reactions, Affinity chromatography, Buffers, Centrifugation, Chromatography, Detergents, Dialysis, Fractionation, Ion Exchange, PAGE, Precipitation, Sample preparations, Separation, Size-exclusion chromatography, Tagged proteins

Handling Inclusion Bodies

Recombinant proteins are most often expressed in the intracellular space, but expression can also be controlled so that the protein is secreted into the periplasmic space or out into the culture medi...
Keywords: Affinity chromatography, Buffers, Chromatography, Detergents, Dialysis, Enzyme activity, Fractionation, Gene expression, Immobilization, Mass spectrometry, Nuclear magnetic resonance spectroscopy, PAGE, Purification, Reversed-phase chromatography, Sample preparations, Separation, Size-exclusion chromatography, Solvents, Sonication, Tagged proteins

Manual and Automated Purification

Chapter 2, Extracted from Affinity Chromatography Vol. 2: Tagged Proteins, GE Healthcare, 2016
Keywords: Affinity chromatography, Centrifugation, Chromatography, Gene expression, Tagged proteins

Performing a Cell Lysis of Recombinant Histidine-Tagged Proteins

For optimal conditions for growth, induction, and cell lysis of recombinant histidine-tagged proteins, please refer to established procedures. The following is a general procedure for cell lysis and ...
Keywords: Affinity chromatography, Buffers, Cell disruption, Centrifugation, Chromatography, Filtration, Homogenization, Precipitation, Sample preparations, Sonication, Tagged proteins

Purification of Histidine-Tagged Proteins using HiTrap TALON Crude

HiTrap® TALON® crude is 1 ml and 5 ml HiTrap® columns prepacked with TALON® Superflow. The columns can be operated with either a syringe, a peristaltic pump, or a chromatography system such as ÄKTA. A...
Keywords: Affinity chromatography, Buffers, Cell disruption, Centrifugation, Chromatography, PAGE, Tagged proteins

Purification of Histidine-Tagged Proteins using TALON Superflow

TALON® Superflow consists of highly cross-linked agarose beads with an immobilized chelating group precharged with Co2+ ions.
Keywords: Affinity chromatography, Buffers, Cell disruption, Chromatography, Filtration, Gene expression, Homogenization, Immobilization, Sample preparations, Sonication, Tagged proteins

Troubleshooting Guide for Affinity Chromatography of Tagged Proteins

The troubleshooting guide below addresses problems common to the majority of purification products discussed in this chapter, as well as problems specific to a particular method. In the latter case, th...
Keywords: Addition reactions, Affinity chromatography, Buffers, Cell culture, Cell disruption, Centrifugation, Chromatography, Detergents, Fermentation, PAGE, Precipitation, Protein biosynthesis, Size-exclusion chromatography, Sonication, Tagged proteins, Western blot

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