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GE28-9538-05

HiTrap® TALON® Crude

GE Healthcare, 28-9538-05, pack of 100 × 1 mL

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Properties

shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging   pack of 100 × 1 mL
mfr. no.   GE Healthcare, 28-9538-05
parameter   3 bar
  5 ml/min - 20 ml/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
column I.D.   16 mm
matrix   6% cross-linked agarose
particle size   60-160 μm
cleaning   2 - 14 (Co2+-stripped medium)
working range   3 - 12 (Co2+-stripped medium)
capacity   ≥20 mg binding capacity (histidine-tagged protein)(The binding capacity for individual proteins may vary)
storage temp.   2-8°C

Description

General description

HiTrap® Talon® crude 1 mL and 5 mL columns allow for rapid separations and easy scale-up with a minimum of sample preparation and equipment.

Application

Talon® Superflow is a cobalt-based IMAC medium offering different selectivity compared to nickel-charged media. Talon® Superflow and prepacked HiTrap® Talon® crude columns allow Protein purification under native or denaturing conditions and can be used with prokaryotic and eukaryotic expression systems.

HiTrap® Talon® crude columns allow for rapid separations and easy scale-up. Samples can be applied directly to the column after thorough cell disruption - without precentrifugation and filtration. The filters in the top and bottom of the column make it possible to directly load unclarified lysates on the column without causing back pressure problems or leakage of the Talon® Superflow beads. Prior to loading samples on HiTrap® Talon® crude columns, cell lysate should be prepared using methods such as sonication, homogenization, or freeze/thaw.

Features and Benefits

• Columns prepacked with cobalt-based chromatography media designed for purification of histidine-tagged recombinant Proteins by immobilized metal affinity chromatography (IMAC).
• Suitable for IMAC purification of histidine-tagged Proteins when Ni2+ is not the optimal choice of metal ion.
• Compatible with commonly used IMAC reagents and appropriate for purifying Proteins under native or denaturing conditions.
• Prepacked HiTrap® Talon® crude columns save time by allowing the application of unclarified samples directly, minimizing labor and degradation of Proteins.
• HiTrap® Talon® crude columns allow repeated runs without stripping, cleaning, or Co2+ recharging.

Analysis Note

www.gelifesciences.com.

Legal Information

HiTrap is a registered trademark of Cytiva

Talon is a registered trademark of Clontech

Safety & Documentation

Safety Information

Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
UN1170 - class 3 - PG 3 - Ethanol, solution

Documents

Certificate of Analysis (COA)

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Protocols & Articles
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