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GE28-9670-70

Protein G Mag Sepharose® Xtra

Cytiva, 28-9670-70, pack of 5 × 1 mL

  •  NACRES NA.56

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Properties

Related Categories GE Healthcare Life Sciences Protein Sample Preparation Formats, Mag Sepharose Magnetic Device, Molecular Biology, Native Protein Sample Preparation, Proteomics More...
shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging   pack of 5 × 1 mL
mfr. no.   Cytiva, 28-9670-70
parameter   Room temperature temp. range
matrix   paramagnetic, spherical, highly cross-linked agarose particles
capacity   27 mg binding capacity(human IgG/ml gel)
storage temp.   2-8°C

Description

General description

protein G Mag Sepharose® Xtra magnetic beads are designed for rapid, small-scale purification and screening of monoclonal and polyclonal antibodies from serum and cell supernatants. The high capacity, > 27 mg human IgG /mL medium allow for the efficient capture of the antibody with high yield and purity. Approximately 350 μg purified human IgG can be obtained from each purification run.

Application

Protein G Mag Sepharose® Xtra magnetic beads combine proven affinity chromatography methods with the magnetic bead platform to improve simplification and ease-of-use in sample preparation. Mag Sepharose® beads are dense, black and visible, and this makes them simple to handle. Less smearing effects and aggregate formations of the magnetic beads mean that no detergents are needed in the buffers.

Together with MagRack 6, a separation tool for handling the beads in microcentrifuge tubes, up to six samples can be processed in parallel. You can easily screen a larger number of samples in parallel with high throughput on a robotic device. Protein G Mag Sepharose® Xtra is available in a 2 × 1 mL pack for 20 purifications and in a 5 × 1 mL pack for 50 purifications. The product is based on Sepharose® medium with magnetite incorporated and the ligand is native Protein G. Protein G has high affinity for the Fc region of IgG antibody from a variety of species.


Protein G Mag Sepharose® is another product in the Mag Sepharose® platform which is designed for the rapid capture and enrichment of Protein samples from cell lysates and biological fluids using immunoprecipitation technology. Protein G Mag Sepharose® is made with an optimized ligand for immunoprecipitation.

Features and Benefits

• Efficient, high capacity small-scale purification and screening of antibodies from various species; 27 μg human IgG/μL magnetic beads
• High purity and yield
• Robust parallel screening of antibodies with high reproducibility
• Simple capture of antibodies from small or large sample volumes (low-microliter to high-milliliter scale)
• Visible and dense Sepharose® based magnetic beads give increased simplicity for handling
• Non-adherent beads eliminate smearing effects and aggregate formation. Can be used without detergents.

Analysis Note

www.gelifesciences.com.

Legal Information

Sepharose is a registered trademark of Cytiva

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer ...
Keywords: Absorption, Affinity chromatography, Buffers, Centrifugation, Chromatography, Detergents, Dialysis, Electrophoresis, Fractionation, Gel electrophoresis, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, PAGE, Precipitation, Sample preparations, Separation, Size-exclusion chromatography, Titrations

Immunoprecipitation Techniques

Appendix 3, Extracted from Affinity Chromatography Vol. 1: Antibodies, GE Healthcare, 2016  
Keywords: Affinity chromatography, Buffers, Cell disruption, Chromatography, Detergents, Electrophoresis, Enzyme activity, Gel electrophoresis, Homogenization, Immobilization, Immunoprecipitation, Mass spectrometry, PAGE, Sample preparations, Separation, Sonication, Western blot

Performing a Purification of IgG Antibodies with Protein A Mag Sepharose Xtra or Protein G Mag Sepharose Xtra

Protein A Mag Sepharose® Xtra and Protein G Mag Sepharose® Xtra products are magnetic beads designed for high capacity small-scale purification/screening of monoclonal and polyclonal antibodies from v...
Keywords: Affinity chromatography, Buffers, Chromatography, Immunoprecipitation, Sample preparations, Separation

Purification using protein A chromatography media

Protein A is derived from a strain of Staphylococcus aureus and contains five regions that bind to the Fc region of IgG. As an affinity ligand, protein A is coupled to Sepharose® so that these region...
Keywords: Affinity chromatography, Buffers, Chromatography, Fermentation, Immunoprecipitation, Ligands, Purification, Size-exclusion chromatography

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