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GE28-9673-88

His Mag Sepharose® Ni

GE Healthcare, 28-9673-88, pack of 2 × 1 mL

  •  NACRES NA.56

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Properties

Related Categories GE Healthcare Life Sciences Protein Sample Preparation Formats, Mag Sepharose Magnetic Device, Molecular Biology, Native Protein Sample Preparation, Proteomics More...
shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging   pack of 2 × 1 mL
mfr. no.   GE Healthcare, 28-9673-88
parameter   Room temperature temp. range
matrix   paramagnetic, spherical, highly cross-linked agarose particles
capacity   50 mg binding capacity(histidine-tagged protein/ml medium)

Description

General description

His Mag Sepharose® Ni is magnetic beads designed to simplify small-scale purification and expression screening of histidine-tagged proteins.

Application

His Mag Sepharose® Ni products are magnetic beads designed for rapid, small-scale purification and screening of histidine-tagged Proteins from different sources. Mag Sepharose® format has excellent properties for small-scale experiments. The high density of the beads allows rapid capture by magnetic devices while the visibility of the beads ensures reliable collection of the bound histidine-tagged Proteins in the purification procedure

The product is based on IMAC Sepharose® medium with magnetite incorporated and nickel immobilized.

Together with MagRack 6, a separation tool for handling the beads in microcentrifuge tubes, up to six samples can be processed in parallel

Features and Benefits

• High capacity magnetic beads for small-scale purification and screening of histidine-tagged Proteins
• Easy to use visible and dense magnetic beads
• High purity and yield
• Easy parallel screening with high repeatability
• Scalable-simple capture of target Protein from small μL to high mL sample volumes

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

www.gelifesciences.com.

Legal Information

Sepharose is a registered trademark of Cytiva

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Protocols

Characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and Uncharged IMAC Sepharose Products

Appendix 1, Extracted from Affinity Chromatography Vol. 2: Tagged Proteins, GE Healthcare, 2016  
Keywords: Affinity chromatography, Buffers, Cell culture, Centrifugation, Chromatography, Detergents, Immobilization, Precipitation, Purification, Tagged proteins

Magnetic Bead-Based Purification/Screening Using His Mag Sepharose Ni

His Mag Sepharose® Ni is a magnetic-bead-based IMAC medium charged with nickel ions. It is designed for efficient, small-scale purification/screening of histidine-tagged proteins from various sources. ...
Keywords: Affinity chromatography, Buffers, Cell culture, Chromatography, Immobilization, Separation, Tagged proteins

Magnetic Bead-Based Purification of Histidine-Tagged Proteins Secreted into Eukaryotic Cell Culture Supernatants Using His Mag Sepharose Excel

His Mag Sepharose® excel is a magnetic-bead-based IMAC medium charged with nickel ions. The magnetic beads are designed for simple, small-scale capture and purification of histidine-tagged proteins se...
Keywords: Affinity chromatography, Cell culture, Chromatography, Degradations, Ligands, Separation, Tagged proteins

Manual and Automated Purification

Chapter 2, Extracted from Affinity Chromatography Vol. 2: Tagged Proteins, GE Healthcare, 2016
Keywords: Affinity chromatography, Centrifugation, Chromatography, Gene expression, Tagged proteins

Purification of Histidine-Tagged Recombinant Proteins Using Ni Sepharose 6 Fast Flow

Ni Sepharose 6 Fast Flow consists of 90 µm beads of highly cross-linked agarose, to which a chelating ligand has been immobilized and subsequently charged with Ni2+ ions. The ligand density of Ni Sep...
Keywords: Affinity chromatography, Buffers, Cell disruption, Chromatography, Filtration, Homogenization, Immobilization, Ligands, Sample preparations, Sonication, Tagged proteins

Troubleshooting Guide for Affinity Chromatography of Tagged Proteins

The troubleshooting guide below addresses problems common to the majority of purification products discussed in this chapter, as well as problems specific to a particular method. In the latter case, th...
Keywords: Addition reactions, Affinity chromatography, Buffers, Cell culture, Cell disruption, Centrifugation, Chromatography, Detergents, Fermentation, PAGE, Precipitation, Protein biosynthesis, Size-exclusion chromatography, Sonication, Tagged proteins, Western blot

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