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GE28-9782-44

HiScreen Ni Fast Flow

Cytiva, 28-9782-44

  •  NACRES NA.56

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Properties

shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging   pkg of 1 ea
mfr. no.   Cytiva, 28-9782-44
parameter   1.5 bar (22 psi) (Over the Packed Bed During Operation)
  22 psi
bed size   7.7 mm × 100 mm
bed volume   4.7 mL
column I.D.   7.7 mm
matrix   6% cross-linked agarose
particle size   45-165 μm
average diameter   90 μm
cleaning   2 - 14 (Ni2+-stripped medium.)
working range   3 - 12 (Ni2+-stripped medium.)
capacity   ~40 mg binding capacity(histidine-tagged protein)
suitability   suitable for bioprocess medium

Description

General description

HiScreen columns prepacked with Ni Sepharose® 6 Fast FLow for method optimization and parameter screening for purification of histidine-tagged proteins with Immobilized Metal ion Affinity Chromatography (IMAC).

Application

HiScreen columns are part of the process development platform available from Cytiva. The columns are prepacked with a range of BioProcess chromatography media (resin) and designed for method optimization and parameter screening. HiScreen columns have small bed volumes (4.7 ml), reducing the cost of sample and buffer consumption. The chromatography media used in HiScreen columns are also available in other prepacked formats and as bulk packs, for all scales of work from development and pilot studies to routine production.

HiScreen Ni FF is prepacked with the BioProcess chromatography medium Ni Sepharose® 6 Fast FLow which has high binding capacities for
histidine-tagged Proteins and minimal Ni2+ leakage.

Features and Benefits

• High binding capacity, approx. 40 mg/mL medium and negligible leakage of Ni2+.
• Prepacked, ready-to-use columns for convenient process development.
• Excellent choice for method optimization and parameter screening due to the 10 cm bed height.
• Easy connection in series to achieve 20 cm bed height.
• Small bed volume gives fast results and minimal sample/buffer consumption
• Reproducible results, scalable to BioProcess columns packed with the same media using the same linear fluid velocity

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

www.gelifesciences.com.

Legal Information

HiScreen is a trademark of Cytiva

Sepharose is a registered trademark of Cytiva

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Protocols

Characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and Uncharged IMAC Sepharose Products

Appendix 1, Extracted from Affinity Chromatography Vol. 2: Tagged Proteins, GE Healthcare, 2016  
Keywords: Affinity chromatography, Buffers, Cell culture, Centrifugation, Chromatography, Detergents, Immobilization, Precipitation, Purification, Tagged proteins

Condition screening for scaling up using HiScreen™ Ni FF

HiScreen Ni FF is a ready-to-use column for purification of histidine-tagged recombinant proteins by IMAC. The column has a volume of 4.7 ml of Ni Sepharose 6 Fast Flow and is well suited for screenin...
Keywords: Affinity chromatography, Buffers, Chromatography, PAGE, Tagged proteins

Desalting/Buffer Exchange and Concentration for Affinity Chromatography of Tagged Proteins

Desalting at laboratory scale is a well-proven, simple, and very fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired bu...
Keywords: Absorption, Addition reactions, Affinity chromatography, Buffers, Centrifugation, Chromatography, Detergents, Dialysis, Fractionation, Ion Exchange, PAGE, Precipitation, Sample preparations, Separation, Size-exclusion chromatography, Tagged proteins

Purification of Histidine-Tagged Recombinant Proteins Using Ni Sepharose 6 Fast Flow

Ni Sepharose 6 Fast Flow consists of 90 µm beads of highly cross-linked agarose, to which a chelating ligand has been immobilized and subsequently charged with Ni2+ ions. The ligand density of Ni Sep...
Keywords: Affinity chromatography, Buffers, Cell disruption, Chromatography, Filtration, Homogenization, Immobilization, Ligands, Sample preparations, Sonication, Tagged proteins

Troubleshooting Guide for Affinity Chromatography of Tagged Proteins

The troubleshooting guide below addresses problems common to the majority of purification products discussed in this chapter, as well as problems specific to a particular method. In the latter case, th...
Keywords: Addition reactions, Affinity chromatography, Buffers, Cell culture, Cell disruption, Centrifugation, Chromatography, Detergents, Fermentation, PAGE, Precipitation, Protein biosynthesis, Size-exclusion chromatography, Sonication, Tagged proteins, Western blot

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